Key Difference – Giemsa Stain vs Wright Stain
In the context of microscopy, staining is considered as an essential step during enhancement of the contrast of the microscopic image, especially to highlight different structures in biological tissues. During the staining of peripheral blood andbone marrow使用涂片,赖特和吉姆萨污渍。这些污渍被称为Romanowsky污渍。这两种污渍都由重要组成部分组成:氧化的亚甲基蓝,曙红Y和Azure B染料。亚甲基蓝色和Azure B的功能是染色细胞核,颜色从蓝色到紫色不等。这些污渍在研究红细胞形态和差异性能过程中广泛使用white blood cellcounts. Diagnosis of different disease conditions such asleukemia可以通过Romanowsky染色程序来实现。Wright staining is used to differentiate blood cells which consist of a mixture of eosin and methylene blue dyes. Giemsa staining is utilized during the staining of bacterial cells as well as human cells and could be combined with Wright stain to develop Giemsa Wright stain.This is the key difference between Giemsa stain and Wright stain.
CONTENTS
- Overview and Key Difference
- 什么是giemsa污渍
- What is Wright Stain
- Giemsa污渍和赖特污渍之间的相似之处
- 并排比较 - GIEMSA染色与Wright污渍以表格形式
- Summary
什么是Giemsa污渍?
GIEMSA染色用于细胞遗传学和组织病理学诊断parasitesofmalaria和其他寄生虫病。吉姆沙染色剂可以肌萎缩性侧索硬化症o be considered as a basic stain in classifyinglymphomasin the classification of Kiel. Giemsa stain is needed for Giemsa banding which is commonly known as G-banding. Giemsa banding is used to stain chromosomes and also used in creating karyograms. Chromosomal abnormalities like translocations and rearrangements are identified through Giemsa banding. Giemsa stain is used in histology due to its high-quality staining of the nuclear membrane andchromatin,基于细胞类型的某些细胞成分的金属菌素以及细胞质染色的不同质量。
GIEMSA溶液含有亚甲基蓝,Azure B和曙红,并使用GIEMSA粉末商业制备污渍。污渍的稳定性取决于亚甲基蓝烯及其混合物以及甲基蓝色的甲基蓝色。Giemsa污渍特别适合phosphateDNA链中的组,并附着在存在大量腺嘌呤 - 胸腺嘧啶键的区域。在GIEMSA染色方法中,最初将样品的薄层放在微观幻灯片上,几滴纯甲醇大约30秒。然后将载玻片浸入5%的GIEMSA染色溶液中,该溶液是新鲜制备的,约20至30分钟。最后,将滑梯用自来水洗涤,然后干燥。Giemsa污渍被称为差异污渍,因为赖特的giemsa染色是在赖特的污渍与吉姆萨(Giemsa)结合使用时形成的。因此,它可用于研究与人类细胞附着的致病细菌。在这里,人类细胞和bacterial cellsare stained deferentially and purple and pink colors are observed respectively。
What is Wright Stain?
赖特(Wright)的污渍以詹姆斯·荷马·赖特(James Homer Wright)的名字命名,他修改了罗曼诺夫斯基污渍。赖特(Wright)的污渍用于区分血细胞类型,因为它有助于区分血细胞类型。结果,可以通过观察白细胞计数来诊断感染。污渍是曙红的混合物,红色是红色的,甲基蓝色的染料。赖特(Wright)的污渍用于染色和观察尿液样品,外周血涂片和骨髓在光显微镜下抽吸。赖特(Wright)的染色用于染色细胞遗传学中的染色体,以促进几种疾病和综合征的诊断。用赖特的污渍染色的尿液样品鉴定出嗜酸性粒细胞表明尿道感染。
在赖特(Wright)的污渍过程中,准备了风干膜,并施加了赖特污渍并将其保留3分钟。然后,添加等量的污渍缓冲液,轻轻混合并剩下5分钟。载玻片水平固定,并用中性蒸馏水充分洗涤。最后,将其干燥并在显微镜下观察。
GIEMSA污渍和赖特污渍之间有什么相似之处?
- 这两种污渍都由重要组成部分组成:氧化的亚甲基蓝,曙红Y和Azure B染料。
- Both are used during the performing of differential white blood cell counts and study of cell morphology of red blood cells.
- Both are differential stains.
What is the Difference Between Giemsa Stain and Wright Stain?
Giemsa污渍vs Wright Stain |
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Giemsa stain is a differential staining technique used primarily for staining of bacterial cells and also human cells. | 赖特染色是一种主要用于血液涂片,尿液样品和骨髓抽吸物的染色程序。 |
Summary – Giemsa Stain vs Wright Stain
染色是一种必不可少的实验室技术,在显微镜过程中使用,用于增强显微镜图像的对比度。GIEMSA染色和赖特污渍共同称为Romanowsky污渍,涉及进行差异白细胞计数以及对红细胞细胞形态的研究。氧化的甲基蓝,曙红Y和Azure B染料是Romanowsky污渍的重要组成部分。首先,在细菌细胞的染色过程中使用GIEMSA染色,但也可以用于人类细胞。在血液涂片,尿液样品和骨髓抽吸物的染色期间,赖特染色被广泛使用。这是Giesma污渍和赖特的污渍之间的区别。
下载GIEMSA染色与Wright污渍的PDF版本
您可以下载本文的PDF版本,并根据引文注释将其用于离线目的。请在此处下载PDF版本GIEMSA污渍和赖特污渍之间的区别
参考:
1. Barcia, J J. “The Giemsa stain: its history and applications.” International journal of surgical pathology., U.S. National Library of Medicine, July 2007,在这里可用。2017年9月12日访问
2. Krafts, K P, and S E Pambuccian. “Romanowsky staining in cytopathology: history, advantages and limitations.” Biotechnic & histochemistry: official publication of the Biological Stain Commission., U.S. National Library of Medicine, Apr. 2011,在这里可用。2017年9月12日访问
图片提供:
1. “Trypanosoma-evansi-rat-blood-Giemsa-stain” By Alan R Walker – Own work(CC BY-SA 3.0)通过Commons Wikimedia
2. “Mast Cell, Bone Marrow Aspirate, Wright Stain (5916735712)” By Ed Uthman from Houston, TX, USA – Mast Cell, Bone Marrow Aspirate, Wright StainUploaded by CFCF(CC BY 2.0)通过Commons Wikimedia
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