关键区别 - 基因测序与DNA指纹识别
测序DNA是分子遗传学的重要技术,其中核苷酸特定DNA序列或整体的序列基因组of an organism is determined. This enables the researcher or the diagnostician to determine the突变DNA序列的基础,并根据其遗传组成区分另一种生物。Gene sequencing is the sequencing procedure of a gene or a DNA fragment viaSanger测序or下一代测序。DNA fingerprinting involves a technique known as Restriction Fragment length polymorphism (RFLP),其中两个或多个受试者的DNA样本被分散并分析以确定一个人的身份。This is the key difference between gene sequencing and DNA fingerprinting.
CONTENTS
1。概述和关键差异
2。什么是基因测序
3。What is DNA Fingerprinting
4。基因测序和DNA指纹之间的相似性
5.Side by Side Comparison – Gene Sequencing vs DNA Fingerprinting in Tabular Form
6.Summary
什么是基因测序?
进行基因测序以确定特定基因的核苷酸序列。如果整个基因组被测序,则称为整个基因组测序。最初,使用使用有害化学物质(例如吡啶的有害化学物质)进行基因测序。由于实验的毒性性质,这种技术很快就停止了。目前,基因的测序主要是使用称为Sanger测序的方法进行的,该方法利用脱氧核糖核酸使用链终止步骤。反应是在四个单独的测试管中进行的底漆is labeled using a fluorescence marker which will finally determine the sequence of the particular fragment. Automated Sanger sequencing uses a detector to detect the fluorescence signals and deliver the results.
Next Generation sequencing is the most recent development of sequencing methods and is a high throughput technique which is equivalent to performing 1000 Sanger sequencing reactions at one time. The main features of Next Generation Sequencing are;
- Highly parallel – many sequencing reactions take place at the same time.
- 显微镜 - 反应很小,可以在芯片上立即完成许多反应。
- 快速 - 由于反应是并行完成的,因此结果准备得更快。
- 较短的长度 - 读取通常范围为505050 -700700700的核苷酸。
基因测序主要用于确定新基因的序列或分析患病状态中存在的基因的突变并确认疾病的遗传基础。它也用于农业生物技术领域,以确定植物种类的新品种,并确定负责有益的农艺特征的植物基因,例如耐药性,抗病性和耐旱性。
什么是DNA指纹?
DNA fingerprinting is a technique mainly used in forensic studies to confirm the identity of a person involved in a forensic investigation. In early days, DNA fingerprinting was done using a hybridization technique using fluorescent or radio labeled markers. At present, DNA fingerprinting is done using the technique RFLP. This technique uses限制酶,,,,which are enzymes capable of chopping DNA at specific sequences. When two samples are brought for analysis, both samples are digested with the same restriction enzymes to yield fragments. If the two samples are similar, theelectrophoresis gel对于两个样品,图像都应相同。如果不相似,则凝胶图像将不会相同。因此,可以通过此技术来确认一个人的身份。
DNA fingerprinting is most often used in finding the true suspect of a crime scene by analyzing the available biological samples at the crime scene. DNA is extracted from these available samples (hair/semen/spit/blood) and analyzed with the DNA samples of the suspects to determine the true culprit.
基因测序和DNA指纹之间有什么相似之处?
- 在这两种情况下,分析的样品都是DNA样品。
- Electrophoresis is used to determine the results in both techniques.
What is the Difference Between Gene Sequencing and DNA Fingerprinting?
基因测序与DNA指纹识别 |
|
基因测序是确定特定基因或整个基因组的核苷酸序列的过程。 | DNA指纹涉及一种技术,其中两个或多个受试者的DNA样品被碎裂并分析以确定一个人的身份。 |
基础 | |
Sequencing methods such as Sanger sequencing or Next Generation sequencing are used in gene sequencing. | 限制片段长度多态性用于分析DNA指纹识别中的两个受试者样本。 |
Applications | |
Gene sequencing is mainly used in genetic studies to analyze novel genes, to identify mutations and to develop inferences based on genetic diagnostics. | DNA fingerprinting is used in forensic investigations to derive conclusions about the identity of a suspect. |
摘要 - 基因测序与DNA指纹识别
基因测序和DNA指纹已成为两个流行的测试,这些测试是为了表征特定基因或使用该人的遗传指纹识别特定人的两种测试。这些技术是准确且迅速的,并且由熟练的人员进行,以得出确认的结果。基因测序和DNA指纹识别之间的差异在于,基因测序集中于寻找基因的确切核苷酸顺序,而DNA指纹刻有侧重于确认法医研究中个体的身份。
Download PDF Version of Gene Sequencing vs DNA Fingerprinting
您可以下载本文的PDF版本,并根据引文注释将其用于离线目的。请在此处下载PDF版本基因测序和DNA指纹之间的差异
References:
1.“ DNA指纹印刷。”Britannica.com。在这里可用。2017年9月6日访问。
2.“ DNA指纹识别 - Geneed - 遗传学,教育,发现。”美国国家医学图书馆,国立卫生研究院在这里可用。2017年9月6日访问。
3.“ DNA测序。”。可汗学院,在这里可用。2017年9月6日访问
图片提供:
1。“Radioactive Fluorescent Seq” By Abizar at English Wikipedia(CC BY-SA 3.0)通过下议院维基梅迪亚
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