关键差异 -蛋白质A vs Protein G
The purification ofIgg抗体, their subclasses and other types ofimmunoglobulins(Iga, IgE, IgD andIgM)通常通过使用细菌进行了通常进行的proteinsthat have high affinity towards the Fc region of these antibodies. Protein A and Protein G are bacterial recombinant proteins which are highly recommended for the purpose of purification of human and other animals’ IgG immunoglobulins. And also protein A, protein G, protein A/G and protein L are native microbial recombinant proteins that have specific binding sites for the Fc region of mammalian IgG antibodies. Other than that, these microbial proteins also can be used to purify other immunoglobulin types such as IgA, IgE, IgD, and IgM in mammals and other animals such as rabbit, mouse, sheep, cow etc. Protein A binds to human antibodies other than IgG antibody. But it is weakly binding with human IgG3亚类,它与人类抗体IgD没有结合。蛋白G与人IgG抗体的所有亚型结合,与其他物种的IgG抗体结合时,它更具用途。但是,蛋白质G与IgG以外的其他类型的人类抗体没有结合。蛋白质ghas a higher affinity towards IgG than protein A。This is thekey difference蛋白A和蛋白质G之间。
内容
1.Overview and Key Difference
2.什么是蛋白A
3.什么是蛋白质G
4。蛋白A和蛋白质G之间的相似性
5。并排比较 - 蛋白质A与表格形式的蛋白质G
6。概括
什么是蛋白质A?
蛋白A的定义为42 kDa的表面蛋白。蛋白A最初在细胞壁中发现金黄色葡萄球菌。它是由“水疗”基因编码。蛋白质是再保险gulated by脱氧核糖核酸拓扑,细胞渗透性,以及两个组件系统。这种微生物重组蛋白高度参与生化反应,因为它具有与多种类型的抗体(例如IgG,IgA,IgA,IgE和IgM)结合的能力。因此,这种微生物蛋白用于纯化人类抗体类型。它具有五个同源的“ Ig”结合域,可将其折叠成三螺旋束。每个结构域都能够与许多具有IgG抗体的最著名的哺乳动物物种的免疫球蛋白蛋白结合。蛋白A特异性结合了大多数免疫球蛋白的FC区域的重链。
With regard to human VH3 family proteins, Protein A binds to the Fab region. The recombinant protein A is broader in its ability to bind to other human antibodies (IgA, IgE, IgM) other than IgG antibody. But it is weakly biding with human IgG3亚类,它与IgD人类抗体没有结合。蛋白A也能够与其他物种的IgG抗体结合,例如马,兔子,小鼠,狗,猴子,牛等。
蛋白质A。plays a pivotal role in金黄色葡萄球菌pathogenesis. This protein facilitates the binding of bacteria to the human Von–Willebrand factor coated surface. Thus, it is increasing the bacterial infection efficiency. Protein A also cripples the humanhumoral mediated immunity。这种微生物重组蛋白是通过工业发酵过程产生的。
什么是蛋白质G?
蛋白质gis defined as the immunoglobulin binding protein that specifically expressed by group C and D streptococcal bacteria. It has high affinity towards the Fc and Fab regions of the antibodies. Protein G has approximately 65kDa molecular size.
蛋白G是表面蛋白。由于其与免疫球蛋白的结合亲和力,它用于抗体纯化。蛋白G与人IgG抗体的所有亚型结合,与其他物种的IgG抗体结合时,它更具用途。但它与其他人类抗体类型(IgA,IgE,IgM,IgD)没有结合。蛋白质G - B1域的折叠结果a球状蛋白。
蛋白A和蛋白质G之间有什么相似之处?
- 两者都是微生物蛋白。
- Both are recombinant proteins.
- Both proteins have high affinity towards human IgG antibody and its subclasses.
- Both are used for immunoglobulins purification.
- 两种蛋白质都可以与免疫球蛋白的FC区域结合。
蛋白A和蛋白质G之间有什么区别?
蛋白质A。vs Protein G |
|
蛋白质A。is defined as a 42 kDa size surface protein which is originally found in the cell wall of金黄色葡萄球菌。 | 蛋白质gis defined as a 65kDa size surface immunoglobulin binding protein that specifically expressed by group C and D streptococcal bacteria. |
Expressing Bacterial Type | |
蛋白质A。is expressed by金黄色葡萄球菌 | 蛋白G由C组和D链球菌细菌表达。 |
Molecular Size | |
蛋白A的大小为42KDA。 | 蛋白质ghas approximately 65kDa size (G148 protein G-65kDa and C40 protein G- 58kDa). |
与人血清白蛋白结合 | |
蛋白质A。does not bind to serum白蛋白。 | 蛋白质ghas binding sites for serum albumin. |
人类IgG的纯化3Subclass | |
蛋白A不能用于纯化人IgG3子类,因为它与人IgG没有结合3subclass immunoglobulin. | 蛋白质G可用于纯化人IgG3subclass as it is binding to the human IgG3subclass immunoglobulin. |
纯化其他人类抗体(IgA,IgE和IgM) | |
蛋白质A。has a higher ability to bind to other human antibodies other than IgG antibody. So, it can be used to purify other human antibodies such as; IgA, IgE and IgM. | 蛋白G与人IgG抗体的所有亚型结合。但是蛋白质G与其他人类抗体没有结合;Iga,IgE和IgM。因此,它不能用来净化其他人类抗体,例如;IGA,IGE和IGM |
摘要 - 蛋白质A vs Protein G
The microbial recombinant protein A, protein G, protein A/G and protein L are native bacterial proteins that have specific binding sites for the Fc region of the mammalian IgG antibodies. Protein A and protein G also have binding sites for the other IgG antibodies of other species. Protein A and protein G are bacterial recombinant proteins which are highly recommended for the purpose of purification of human and other animals’ IgG immunoglobulins. Protein L has high affinity towards the kappa light chains of immunoglobulin classes IgG, IgA, and IgM. So, protein L can be incorporated to purify these immunoglobulin types in humans and other species. Industrially all these bacterial proteins are using currently to purify immunoglobulins like; IgG, IgA, IgD, IgE, and IgM. This can be described as the difference between Protein A and Protein G.
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Reference:
1.“蛋白质A”Wikipedia,Wikimedia基金会,2017年12月26日。Available here
2.“Protein G.” Wikipedia, Wikimedia Foundation, 16 Dec. 2017.Available here
Image Courtesy:
1.蛋白a 1dee 1l6x'by e a s - 自己的工作,(CC BY-SA 3.0)viaCommons Wikimedia
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