正向和反向遗传学之间的差异

Key Difference – Forwardvs反向遗传学

With the development of modern technology, genetically related techniques have been developed along the same vector that led to the basis of modern molecular biology. Different techniques under this category could be explained. Such techniques are used in the process of determination and investigation of different genomic traits of living organisms. Forward and reverse genetics are such techniques in the context of the above processes.正向遗传学是确定造成特定遗传学基础的途径表型。Reverse genetics is a technique that is utilized to investigate and understand the function of a particular基因或a基因序列通过对基因产生的表型的分析。这是关键区别between forward and reverse genetics.

CONTENTS

1。概述和关键差异
2。什么是前瞻性遗传学
3。什么是反向遗传学
4。前向遗传学和反向遗传学之间的相似之处
5.Side by Side Comparison – Forward vs Reverse Genetics in Tabular Form
6.Summary

什么是前瞻性遗传学？

正向遗传学可以定义为确定基础的途径基因ticsthat is responsible for a particular phenotype. Naturally occurring突变由辐射，化学物质或可转座元素（插入诱变）诱导的突变体是正向遗传学的初始方法。然后是繁殖，突变个体的隔离和基因的映射。通过分析改变的DNA序列的表型效应来确定基因功能。因此，它被认为是反向遗传学的拮抗作用。通常会事先检查突变表型，以鉴定负责的特定基因，并可能引起以各自突变表型命名的基因。果蝇红润以突变体的眼睛命名的基因就是一个例子。

In the context of conventional genetical approach, a researcher handling the determination process of the genetic basis for phenotypes would directly map the gene on the particular chromosome where it is present. This is done through cross-breeding with different individuals where those individuals carry different other uncommon traits. Statistical analysis will be carried out to determine the frequency of occurrence where the two traits are inherited together. This convention methodology of mapping takes a considerable long period of time.

什么是反向遗传学？

在反向遗传学的背景下,这是一个技术que that is utilized to investigate and understand the function of a particular gene or a gene sequence through the analysis of the phenotype that is generated by the gene. This methodology is the total opposite of the concept forwards genetics. With the intention of learning the influence of a particular sequence upon its phenotype or to investigate the biological function of it, the modern researches tamper theDNA序列他们在序列中设计特定更改或破坏它的地方。一旦对DNA序列进行了故意更改，研究人员将观察到其结果会发生的表型变化。遗传序列的故意改变是通过不同方法和遗传技术来完成的。这些技术包括定向缺失，点突变，基因沉默和转基因的使用。

In directed deletions andpoint mutations，，，，a site-directed mutagenesis is induced. A site-directed mutagenesis refers to the fact where a change is induced through a mutation in regulatory regions of a发起人一个基因。通过在开放阅读框中诱导密码子变化，也可以实现位置定向的诱变。该技术还用于开发其创建非功能基因的无效等位基因。可以通过使用来实现基因沉默RNAi (RNA interference)。这是一个双链RNA，将针对特定的RNAmRNAand disrupts it and thereby prevent the translation process. Hence, the phenotype is not expressed since the particular protein is not produced.

What is the Similaritiy Between Forward and Reverse Genetics?

• 前进和反向遗传学现象均针对特定表型的遗传。

What is the Difference Between Forward and Reverse Genetics?

Forward vs Reverse Genetics
正向遗传学可以定义为确定基础的途径基因tics responsible for a particular phenotype.	反向遗传学是一种通过分析该基因产生的表型来研究和理解特定基因或基因序列的功能的技术。

Summary – Forwardvs反向遗传学

正向遗传学可以定义为确定负责特定表型的遗传学基础的途径。由辐射，化学物质或可转座元素（插入诱变）诱导的自然发生的突变和突变体是正向遗传学的初始方法。通过分析改变的DNA序列的表型效应来确定基因功能。反向遗传学是一种通过分析该基因产生的表型来研究和理解特定基因或基因序列的功能的技术。遗传序列的故意改变是通过不同方法和遗传技术来完成的。这些技术包括定向缺失，点突变，基因沉默以及转基因的使用等。这是正向遗传和反向遗传学之间的差异。

参考：

1。Griffiths, Anthony JF. “Reverse genetics.” An Introduction to Genetic Analysis. 7th edition., U.S. National Library of Medicine, 1 Jan. 1970.在这里可用
2。Takahashi, Joseph S., et al. “Forward and Reverse Genetic Approaches to Behavior in the Mouse.” Science (New York, N.Y.), U.S. National Library of Medicine, 17 June 1994.在这里可用

图片提供：

1. eyversegeneticsflu’by mouagip - 通过下议院维基梅迪亚