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Key Difference – Direct vs Indirect Coombs Test Coombs test is a type of blood test used to diagnose anemia conditions. It detects the presence of some antibodies produced by the immune system. These antibodies are able to destroy red blood cells in the blood, causing low red blood cell counts. Hence, the presence […]
Key Difference – Direct vs Indirect ELISA An enzyme-linked immunoassay (ELISA), also known as enzyme immunoassay, is a serological test which detects antibodies in the blood. It is used as a diagnostic tool to find out whether the patient has been exposed to a particular type of virus or another infectious agent (antigen) and whether […]
Key Difference – Nitrocellulose vs PVDF Western blotting is a method which allows detection and quantification of specific proteins from a protein sample. Reliability of the technique depends on the selection of a correct membrane for absorption of proteins from the gel. There are different types of microporous membranes. Nitrocellulose and PVDF membranes are […]
Key Difference – Base Excision Repair vs Nucleotide Excision Repair DNA is frequently subjected to damages due to various internal and external factors. However cellular repairing systems immediately and constantly correct the damages before they become mutations or before they are transferred to succeeding generations. There are three types of excision repairing systems in […]
Key Difference – YAC vs BAC Vectors Vectors are used in molecular cloning. A vector can be defined as a DNA molecule which behaves as a vehicle to carry foreign genetic material into another cell. A vector containing foreign DNA is known as recombinant DNA and it should have the capability of replicating and expressing it within the host organism. Yeast artificial […]
Key Difference – SDS Page vs Western Blot Western blotting is a technique which detects a specific protein from a protein sample. This technique is performed via several key steps: gel electrophoresis, blotting, and hybridization. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS Page) is a kind of gel electrophoresis technique which is used to […]
Key Difference – Northern vs Southern vs Western Blotting Detection of specific sequences of DNA, RNA, and proteins is essential for various types of studies in Molecular biology. Gel electrophoresis is a technique which separates DNA, RNA, and proteins according to their sizes. From the gel profiles, particular DNA sequence, RNA sequence, or protein […]
Key Difference – Transient vs Stable Transfection Transfection is a process which is involved in the gene transfer of eukaryotic cells using chemical or physical methods. Transfection can be classified into main two types named transient transfection and stable transfection. During transient transfection, the gene of interest fails to integrate with the host genome […]
Key Difference – Selectable Marker vs Reporter Gene Genetic engineering technique is used to transfer important genes from organism to another organism. Insertion of a foreign gene into another organism’s genome and making it expressed within the host cell are most difficult steps in gene transferring. To detect the success of the transformation process, […]
Key Difference – Transfection vs Transduction In biotechnology and genetic engineering, foreign genes are introduced into genomes of the organisms with the objective of improving characteristics of the organisms. There are physical, chemical and biological methods available to introduce foreign DNA into host cells. Transfection and transduction are two types of gene transferring methods […]
