Thekey differencebetween guanidine thiocyanate and guanidine hydrochlorideis that guanidine thiocyanate is a stronger protein denaturant that is more commonly used in RNA isolation, while guanidine hydrochloride is a weaker protein denaturant that is less commonly used in RNA isolation.
Denaturation是蛋白质失去其第四纪结构,第三结构和二级结构的过程,通常存在于其本地状态。可以通过应用某些外部应力或化合物,例如浓酸或碱,浓浓度无机盐,有机溶剂(酒精,氯仿),搅拌,辐射或热量来完成。鸟嘌呤硫氰酸盐和盐酸盐酸盐是两种不同类型的蛋白质变性剂。
内容
1.Overview and Key Difference
2.什么是鸟嘌呤硫氰酸盐
3.What is Guanidine Hydrochloride
4.Similarities – Guanidine Thiocyanate and Guanidine Hydrochloride
5.Guanidine Thiocyanate vs Guanidine Hydrochloride in Tabular Form
6.Summary – Guanidine Thiocyanate vs Guanidine Hydrochloride
什么是鸟嘌呤硫氰酸盐?
Guanidine thiocyanate (GTC) is a stronger protein denaturing agent that is more commonly used in RNA isolation. It is also known asguanidinium isothiocyanate(GITC)。成为一个chaotropic agent, it is a chemical compound routinely used as a general protein denaturant. A chaotropic agent is a molecule in a water solution that can disrupt thehydrogen bondingnetwork between water molecules. This has an effect on the stability of the native state of other molecules in the solution, such as macromolecules like proteins andnucleic acids通过削弱疏水作用。像鸟胺硫氰酸盐这样的混合物剂减少了由疏水性氨基酸周围的水分子和水合壳形成的蛋白质结构中的顺序量。这可能会导致蛋白质变性。
Guanidine thiocyanate can be used to deactivate viruses such as influenza that cause diseases like the Spanish flu in 1918. Therefore, it can be used in medical or hospital setups. Furthermore, Guanidine thiocyanate is also used to lyse cells and virus particles in RNA and DNA extractions. Here, the function of guanidine thiocyanate is to aid lysing action and to prevent the activity of RNase enzymes and DNase enzymes by denaturing. These enzymes would otherwise damage the extract (RNA or DNA).
What is Guanidine Hydrochloride?
Guanidine hydrochloride (GdnHCl) is a weaker protein denaturant that is less commonly used in RNA isolation. It is also known asguanidinium chloride(GdmCl). It is the hydrochloride salt of guanidine. Guanidine hydrochloride is chaotropic and one of the denaturants used in the physicochemical studies of protein folding.
Guanidine hydrochloride also has the ability to decrease enzyme activity and increase the solubility of hydrophobic molecules. Normally, at higher concentrations of guanidine hydrochloride, proteins lose their ordered structure. Proteins tend to become randomly coiled at this concentration of guanidine hydrochloride. In medical or hospital setups, guanidine hydrochloride is indicated for the reduction of the symptoms of muscle weakness and easy fatigability associated with the disease Eaton-Lambert syndrome. Furthermore, side effects of using guanidine hydrochloride may include peristalsis, diarrhoea, and fatal bone marrow suppression.
鸟嘌呤硫氰酸盐和盐酸鸟嘌呤之间有什么相似之处?
- 鸟嘌呤硫氰酸盐和盐酸盐酸盐是两种不同类型的蛋白质变性剂。
- Both denaturants have guanidine in their structures.
- They are chaotropic agents.
- Both denaturants can be used for RNA isolation.
- 它们都用于医疗或医院设置。
鸟嘌呤硫氰酸盐和盐酸盐碱有什么区别?
鸟嘌呤硫氰酸酯是一种较强的蛋白质变性剂,更常用于RNA分离中,而盐酸鸟嘌呤盐酸盐是一种较弱的蛋白质变性剂,在RNA分离中不太常用。因此,这是鸟嘌呤硫氰酸盐和盐烷盐酸盐之间的关键差异。此外,鸟胺硫氰酸盐的化学式为c2H6N4S, while the chemical formula of guanidine hydrochloride is CH5N3.HCl。
下面的信息图列出了鸟嘌呤硫氰酸硫氰酸盐和盐烷盐酸盐之间的差异,以并排比较.
Summary – Guanidine Thiocyanate vs Guanidine Hydrochloride
鸟嘌呤硫氰酸盐和盐酸盐酸盐是两种不同类型的蛋白质变性剂。Both denaturants are chaotropes. Guanidine thiocyanate is a stronger protein denaturant agent that is more commonly used in RNA isolation, while guanidine hydrochloride is a weaker protein denaturant that is less commonly used in RNA isolation. So, this summarizes the difference between guanidine thiocyanate and guanidine hydrochloride.
Reference:
1.“”Guanidinium Thiocyanate”。一个啊verview | ScienceDirect Topics.
2“Guanidine Hydrochloride。”国家生物技术信息中心。美国国家医学图书馆。
Image Courtesy:
1.“”SPROX” Sgold626 - 自己的工作(CC BY-SA 4.0)via Commons Wikimedia
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