Difference Between Tris Base and Tris HCl

Key Difference – Tris Base vs Tris HCl

Tris base and tris HCl are organic compounds that can be used in the preparation of buffer solutions. There are many similarities as well as differences between tris base and tris HCl. The only difference between the chemical structure of two compounds is that tris HCl has anHClmolecule associated withtris(hydroxymethyl)aminomethanemolecule which is in general known as tris base. The key difference between tris base and tris HCl is thattris base contains the chemical formula C₄H₁₁NO₃whereas tris HCl contains the same chemical formula with an additional HCl molecule.

CONTENTS

1.Overview and Key Difference
2.三羟甲基氨基甲烷基础液是什么
3.What is Tris HCl
4.Similarities Between Tris Base and Tris HCl
5.Side by Side Comparison – Tris Base vs Tris HCl in Tabular Form
6.Summary

三羟甲基氨基甲烷基础液是什么?

The term tris base is used to name the compound tris(hydroxymethyl)aminomethane, having the chemical formula C₄H₁₁NO₃. It is also known asTHAM. It is an organic compound used as a component in the buffer solutions TAE and TBE buffers.

This compound contains a primaryaminegroup. Therefore it can undergo reactions that a typical primary amine undergoes; eg:condensationreactions withaldehydes. The basic properties of this compound arise due to this amine group. The molar mass of this compound is 121.14 g/mol. It is a white crystalline powder at room temperature and pressure. The melting point of this compound is around 176 °C, and the boiling point is 219 °C.

The effective pH range of this compound is between 7.5 to 9.0. This is because thepKaof conjugated acid of this compound is at 25^oC is 8.07. This buffer can inhibit certain enzymes by changing thepH. The compound is prepared industrially by the condensation of nitromethane withformaldehydeunder basic conditions.

What is Tris HCl?

三羟甲基氨基甲烷HCl液is tris hydrochloride having the chemical formula C₄H₁₁NO₃· HCl. The molar mass of this compound is 157.59 g/mol. The IUPAC name of tris HCl is2-amino-2-(hydroxymethyl)propane-1,3-diol hydrochloride. It is also known asTHAM hydrochloride.

It is an organic compound that is often used in buffer solutions such as TAE and TBE. This compound is highly water soluble. This compound works better in a pH range from 7.0 to 9.0. It can be used in the preparation of Laemmli buffer, which is used in SDS-PAGE buffers.

三羟甲基氨基甲烷HCl液的制备是通过混合三with HCl. When tris is mixed with tris HCL, a buffer solution can be obtained, which is very useful to avoid the need of working withstrong acidsorstrong bases.

What are the Similarities Between Tris Base and Tris HCl?

• Both are components in TAE and TBE buffer solutions.
• Both are organic compounds.

What is the Difference Between Tris Base and Tris HCl?

Tris Base vs Tris HCl
Tris base refers to tris(hydroxymethyl)aminomethane that has the chemical formula (C4H11NO3).	三羟甲基氨基甲烷HCl液is tris hydrochloride having the chemical formula C4H11NO3· HCl.
Molar Mass
The molar mass of tris is 121.14 g/mol.	The molar mass of tris HCl is 157.59 g/mol.
IUPAC Name
The IUPAC name of tris is tris(hydroxymethyl)aminomethane.	The IUPAC name of tris HCl is 2-amino-2-(hydroxymethyl)propane-1,3-diol hydrochloride.
pH Range
Tris base works best in the range of 7.5 to 9.0.	三羟甲基氨基甲烷HCl液works best in the range of 7.0 to 9.0.

Summary – Tris Base vs Tris HCl

Tris base and tris HCl are components in different buffer solutions. The key difference between tris base and tris HCl is that tris base contains the chemical formula C₄H₁₁NO₃whereas tris HCl contains the same chemical formula with an additional HCl molecule.

Reference:

1. “Tris.” Wikipedia, Wikimedia Foundation, 18 Feb. 2018,Available here.
2.“三羟甲基氨基甲烷HCl液”。三羟甲基氨基甲烷HCl液– Gold Biotechnology,Available here.
3. “Tromethamine hydrochloride.” National Center for Biotechnology Information. PubChem Compound Database, U.S. National Library of Medicine,Available here.

Image Courtesy:

1. “Tris” By Klaus Hoffmeier – Own work (Public Domain) viaCommons Wikimedia
2.“2-amino-2-(hydroxymethyl)propane-1,3-diol 200” By Emeldir (talk) – Own work (Public Domain) viaCommons Wikimedia