Thekey differencebetween Ki67 and BrdUis that Ki67 is a specific protein commonly used in the detection of增殖细胞在活组织中,而Brdu(溴脱氧尿苷)是合成的核苷通常用于检测活组织中的增殖细胞。
细胞增殖增加细胞数量。通常,它是由细胞分裂和通过细胞死亡的细胞丢失之间的平衡来定义的,或分化. Intumours, cell proliferation is increased. Cell proliferation can be measured through compounds or dyes incorporated into live cells. Cell proliferation can also be measured by detecting the expression of certain proteins called proliferation markers in live cells. Ki67 and BrdU are two types of proliferation markers used to detect cell proliferation.
内容
1.Overview and Key Difference
2.What is Ki67
3.什么是BrdU
4.Similarities – Ki67 and BrdU
5.ki67 vs brdu以表格形式
6。摘要 - KI67 vs brdu
What is Ki67?
Ki67 is a specific protein commonly used in the detection of proliferating cells in living tissues. It is also known as antigen-Ki67 or MKI67. The human gene MKI67 codes this protein. This protein is also associated with ribosomal RNA transcription. Inactivation of antigen Ki67 leads to inhibition of ribosomal RNA synthesis. Ki67 is a protein in cells that increases when the cells prepare to divide into new cells. A staining process can measure the percentage of tumour cells that are positive for KI67. The presence of more positive cells indicates that cells divide and form new cells more quickly. In breast cancer, a result of less than 10% is considered low, 10-20% borderline, and high if more than 20%.
在细胞周期的所有活性阶段(G1,S,G2和M)中存在Ki67。但是它在静息细胞中不存在(g0)。因此,可以评估Ki67的核表达以评估免疫组织化学(IHC)的肿瘤增殖。此外,由于其诊断能力,它是病理实验室通常使用的流行增殖标记。
什么是brdu?
BRDU(溴脱氧尿苷)是一种通常用于检测活组织中增殖细胞的合成核苷。为了增殖,细胞需要在S期产生DNA。因此,评估细胞增殖的可靠方法是DNA合成的测量。通常,这是通过将活细胞与化合物或染料一起孵育来完成的,这些化合物或染料可以很容易地掺入新合成的DNA中。
Thymidine analogues are the most popular compound for incorporation into DNA. Therefore, BrdU is a thymidine analogue that is generally used in the detection of proliferating cells in living tissues. BrdU can be incorporated into the newly synthesized DNA of replicating cells during the S phase. This is because it substitutes thymidine during DNA replication. IHC detection of BrdU incorporation into DNA is a powerful technique to study the cytokinetic of normal and tumour cells. Moreover, BrdU labelling of tumour cells involves steps such as the incorporation of BrdU into DNA and specific detection of incorporated BrdU with anti-BrdU antibodies, etc.
What are the Similarities Between Ki67 and BrdU?
- Ki67 and BrdU are two types of proliferation markers used in the detection of cell proliferation.
- Both markers can be used to differentiate normal and tumour cells.
- 它们可以在S相中标记细胞。
- Both markers show similar expression patterns.
- They show high reliability.
KI67和BRDU有什么区别?
Ki67 is a specific protein that is commonly used in the detection of proliferating cells in living tissues, while BrdU is a synthetic nucleoside that is commonly used in the detection of proliferating cells in living tissues. So, this is the key difference between Ki67 and BrdU. Moreover, Ki67 labels cells in G1, G2, S and M phases of the cell cycle while BrdU labels cells only in the S phase of the cell cycle.
The following infographic lists the differences between Ki67 and BrdU in tabular form for side by side comparison.
摘要 - KI67 vs brdu
Ki67 and BrdU are two types of proliferation markers that are useful in the detection of cell proliferation. Ki67 is a specific protein, while BrdU is a synthetic nucleoside. Thus, this is the key difference between Ki67 and BrdU. Ki67 is able to label the cells in the G1, G2, S and M phases of the cell cycle. In contrast, BrdU labels cells only in the S phase of the cell cycle.
Reference:
1.“”Ki-67 (Protein)”。概述| ScienceDirect主题。
2. “Bromodeoxyuridine”。概述| ScienceDirect主题。
Image Courtesy:
1.“”蛋白质MKI67 PDB 1R21''由EMW - 自己的工作(CC BY-SA 3.0)通过Commons Wikimedia
2. “GFAP and BrdU double labeling in the rat subrganular zone” By Charlotte A. Oomen, Carlos E. N. Girardi, Rudy Cahyadi, Eva C. Verbeek, Harm Krugers, Marian Joëls, Paul J. Lucassen – Opposite Effects of Early Maternal Deprivation on Neurogenesis in Male versus Female Rats(CC BY 2.5)通过Commons Wikimedia
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