Difference Between Progressive and Regressive Staining

Thekey differencebetween progressive and regressive staining is thatin progressive staining, thetissue留在染色溶液中足够长的时间到达所需的endpoint在回归性染色时，组织故意将组织过度染色，直到染料饱和所有组织元素，然后去除染色。

Staining is a technique which highlights and differentiates tissue components and makes it feasible to observe under the microscope. H and E staining is the general tissue staining procedure that is most commonly used inhistology。它使用苏木精和伊红(复染色)。Nuclear staining using haematoxylin can be accomplished with progressive or regressive staining techniques. Some haematoxylin formulations are used in progressive staining. Mayer’s haematoxylin is one example. Progressive stains have a low haematoxylin concentration. Hence, they slowly and selectively stain thechromatin。Some other formulations are used in regressive staining. Harris haematoxylin is used in regressive staining. Regressive stains have a high concentration of haematoxylin; hence, the stain rapidly diffuses over the entire cell.

内容

1.Overview and Key Difference
2.What is Progressive Staining
3.What is Regressive Staining
4.Similarities Between Progressive and Regressive Staining
5.并排比较 - 表格形式的渐进式与回归染色
6.概括

什么是进步性染色？

Progressive staining is a technique that allows the tissue in the staining solution just long enough to reach the desired endpoint. Therefore, frequent monitoring of stain quality is needed to determine the completion of staining. The staining intensity is controlled by the immersing time.

吉尔的血久毒素和梅耶尔的血久毒素本质上是渐进的。对于这两种血性，通常在进行性染色中使用5-10分钟的染色时间。通常，进行性血久毒素浓缩较少。因此，它们缓慢而有选择地染色质。在进行性染色中，血久蛋白主要将染色质染色为所需的强度。因此，它不需要稀酸酒精的分化即可拔出多余的污渍。

什么是回归染色？

Regressive staining is a more rapid staining technique in which the tissue is deliberately over stained until the dye saturates all tissue components. Then the tissue is selectively de-stained until it reaches the correct endpoint. De-staining step is called differentiation. Differentiation is done to remove excess stains. Usually, it is done using dilute acid alcohol.

Harris haematoxylin is the popularly used type of haematoxylin. Ehrlich’s and Delafield’s haematoxylins are also using in regressive staining. Regressive stains have a high concentration of haematoxylin. Hence, they rapidly diffuse the entire cell and over stain the chromatin and cytoplasm. Regressive staining is preferred when very clear differentiation of tissue elements is required.

What are the Similarities Between Progressive and Regressive Staining?

• 进行性和回归染色是两种类型的染色技术。
• 两者都使用称为血久的染料。

What is the Difference Between Progressive and Regressive Staining?

Progressive staining is a slower staining process in which the tissue is left in the staining solution just long enough to reach the desired endpoint. In contrast, regressive staining is a more rapid staining process in which the tissue is deliberately over stained and then de-stained. So, this is the key difference between progressive and regressive staining.

此外，进行性染色和回归性染色之间的另一个显着差异是，进行性染色不需要额外的步骤称为分化，而回归染色则需要分化才能去除多余的污渍。此外，进行性污渍的血久阳道蛋白浓度较低，而回归污渍的血久阳道毒素浓度更高。

The below infographic tabulates more differences between progressive and regressive staining.

概括– Progressive vs Regressive Staining

Progressive staining is a slow staining technique in which the tissue is left in the staining solution just long enough to reach the endpoint. Therefore, over staining is not done is progressive staining. In contrast, regressive staining is a more rapid staining technique in which the tissue is over stained and then de-stained. Progressive staining does not require differentiation (removal of excess stain) while regressive staining requires differentiation in dilute acid alcohol in order to remove excess stain. Progressive stains generally have a low concentration of haematoxylins while regressive stains have more concentration of haematoxylins. Thus, this summarizes the difference between progressive and regressive staining.

Reference:

1.“常规H＆amp;e”。slideshare.net，2020年，Available here。
2.“血久毒素 - 蓝调的故事。”vibath，Available here。

Image Courtesy:

1. “Hämatoxylin” By NEUROtiker (talk) – Own work (Public Domain) viaCommons Wikimedia
2. “H&E Eby Loyola University Chicago” By Jonathan Eby – Own work(CC BY-SA 4.0)viaCommons Wikimedia