这Isoschizomers和Isocaudomers之间的关键区别Isoschizomers识别并裂解相同的限制或识别位点,而等审计剂在同一站点识别略有不同的限制位点和裂解。
限制酶或限制内切酶是一种nucleases that cleave double-stranded DNA. They are able to recognize specific sequences called restriction sites, which are palindromic in origin. Bacteria produce restriction enzymes against foreign pathogens, especially against viruses. It is a form of defence mechanism shown by bacteria. Depending on the structure of the enzyme and nature of cuts, there are three types of restriction enzymes asI型限制酶,II型限制酶,和II型限制酶。此外,根据生化活性和隔离来源,有三种类型的限制酶,作为等chizemers,:neoschizomers和Isocaudomers。
内容
1。概述和关键差异
2。什么是Isoschizomers
3。什么是Isocaudomers
4。相似之处 - Isoschizomers和Isocaudomers
5。Isoschizomers vs Isocaudomers以表格形式
6。摘要 - Isoschizomers vs Isocaudomers
什么是Isoschizomers?
Isoschizomers是一种来自不同来源的限制酶,但识别并裂解了DNA中相同的限制位点。因此,两个Isoschizomer具有相同的识别位点。它们在同一站点上切割,因此它们特定于相同的识别顺序。例如,限制性酶SPHI和BBUI是两个等异形体,在CGTAC/G处识别和裂解。
另一对Isoschizomers是HPAII和MSPI。他们都识别序列5'-CCGG-3'并裂解DNA。而且,bspEI和ACCiii也是来自一个来自芽孢杆菌物种和cin骨calcoaceticus,,,,分别。等异,可以从不同的细菌中进行分离。由于它们来自不同的细菌,因此它们的限制条件可能彼此不同。
什么是Isocaudomers?
Isocaudomers are a type of restriction enzyme that recognizes slightly different sequences, but they cleave at the same site to produce the same ends. Isocaudomers also come from different bacteria. Sau3a and BamHI are a pair of isocaudomers. They produce 5’-GATC-3’ sticky end after cleavage of the same site. Another pair of isocaudomers is NcoI fromNocardia Corallina和Pagi来自假单胞菌Alcaligenes。这se two enzymes also bind at different recognition sequences but cut at the same site and produce the same sticky ends. Therefore, producing cutting ends are compatible with each other, and they can be ligated to one another. XhoI, PspXI and SalI are also isocaudomers.
Isoschizomers和Isocaudomers之间有什么相似之处?
- Isoschizomers和Isocaudomers是两种类型的限制酶。
- 两个Isoschizomer具有相同的消化位点,类似于具有相同消化位点的两个Isocaudomers。
- 它们的起源是原始的。
- 两者都有一个限制识别站点和消化位点。
- 它们产生彼此兼容的末端。
- In addition, these enzymes are useful tools in recombinant DNA technology.
Isoschizomers和Isocaudomers有什么区别?
等异构体是限制酶,在同一限制位点识别相同的DNA序列和裂解,而等核剂是限制性酶,识别略有不同的DNA序列,但通过在同一位点切割而产生相同的粘性末端。因此,这是Isoschizomers和Isocaudomers之间的关键区别。
下面的信息图表列出了Isoschizomers和Isocaudomers之间的差异。
摘要 - Isoschizomers vs Isocaudomers
Isoschizomers are restriction enzymes that recognize the same DNA sequence and cleave at the same site. On the other hand, isocaudomers are restriction enzymes that recognize the slightly different sequences and cleave at the same site producing the same ends. Thus, this is the key difference between isoschizomers and isocaudomers. SphI, BbuI, HpaII and MspI are several examples of isoschizomers while Sau3a and BamHI are two examples of isocaudomers. Both isoschizomers and isocaudomers are naturally produced by bacteria.
参考:
1.“”Isocaudomer。”Wikipedia, Wikimedia Foundation, 13 Oct. 2021.
2.“”限制酶:类型和示例。”《学习》,2018年9月4日。
图片提供:
1.“”ECORV限制性站点” By Ramin Herati – Created with Inkscape (Public Domain) via Commons Wikimedia
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