这key difference between isoschizomers and neoschizomers那是Isoschizomers限制酶具有相同的识别顺序并分裂DNAat the same positions, while neoschizomers are restriction enzymes that have the same recognition sequence but cleave DNA at different positions.
Restriction enzymes or restriction endonucleases are enzymes that cleave DNA into fragments at or near specific recognition sites. They cut DNA at restriction sites. Restriction enzymes are usually classified into five groups based on structure, whether they cut their DNA substrate at their recognition site, and whether the recognition and cleavage sites are separate from one another. More than 3600 restriction endonucleases have already been identified. Approximately 800 restriction enzymes are available commercially. Isoschizomers and neoschizomers are two types of restriction enzymes based on recognition site and cleavage specificity.
CONTENTS
1。概述和关键差异
2。什么是Isoschizomers
3。什么是新chizemers
4。相似之处 - 等chizomers和Neoschizomers
5。Isoschizomers vs Neoschizomers in Tabular Form
6.Summary – Isoschizomers vs Neoschizomers
什么是Isoschizomers?
等异形体是具有相同识别序列并在相同位置裂解DNA的限制酶。这些限制酶具有相同的特异性。识别给定序列的第一个发现的限制酶称为原型,而所有后来识别出识别同一序列的限制性酶称为等级酶。然而,等异形体在站点偏好,反应条件上可能有所不同,methylation敏感性和恒星活动。等异,从不同的细菌菌株中分离出来。因此,它们可能需要不同的反应条件。在某些情况下,只有一对等溶剂中只有一个可以识别甲基化和未甲基化的限制位点。另一方面,其他限制酶只能识别限制位点的未甲基化形式。等异形体的这种特殊特征有助于鉴定限制性位点的甲基化状态,同时将其从细菌状态隔离。
例如,Agei和BSHT1以相同的模式识别并切割5’-A↓CCGGT。HPAII和MSPI是其他Isoschizomers。他们俩都识别序列5’-C↓CGG-3'未经甲基化时。但是,当序列的第二个C被甲基化时,只有MSPI才能识别此序列,而HPAII无法识别它。
什么是Neoschizomers?
th Neoschizomers是限制性内切酶e same recognition sequence but cleave DNA at different positions. In some special molecular biological applications, this is a very helpful feature. The neoschizomers are a subset of isoschizomers. Well-known examples for neoschizomers are SmaI (5’-CCC↓GGG-3’) and XmaI (5’-C↓CCGGG-3’); both recognize 5’-CCCGGG-3’ sequence but cleave them at a different position. Thus, these two restriction enzymes generate different types of ends. In this case, SmaI produces blunt ends, and XmaI produces 5’ protruding ends.
另一个例子是MAEII和尾限制酶对。原型MAEII(A↓CGT)产生具有2碱基5'Extension的DNA片段,而Neoschizomer Tail(ACGT↓)产生具有4-碱基3’延伸的DNA片段。
Isoschizomers和Neoschizomers之间有什么相似之处?
- 等异形体和新旋化体是两种类型的限制酶。
- 这y are predominantly found in prokaryotes.
- 它们是细菌和古细菌中发现的限制性修饰(RM)系统的一部分。
- Both are protein molecules.
- 两者都将DNA切成碎片。
Isoschizomers和Neoschizomers有什么区别?
等异构体是具有相同识别序列并在相同位置裂解的限制酶,而新旋化体是具有相同识别序列但在不同位置切割DNA的限制酶。因此,这是Isoschizomers和Neoschizomers之间的关键区别。此外,Isoschizomers具有相同的特异性,而新chizemers具有不同的特异性。
以下信息图列出了Isoschizomers和Neoschizomers以表格形式进行并排比较的差异。
Summary – Isoschizomers vs Neoschizomers
限制酶是DNA切割酶。它们也称为分子剪刀。有不同类型的限制酶。基于识别位点和裂解特异性,限制酶是两种类型,如Isoschizomers和Neoschizomers。等异形体具有相同的识别序列,并在相同的位置上裂解DNA,而新旋化剂具有相同的识别序列,但在不同位置切割DNA。因此,这是Isoschizomers和Neoschizomers之间的关键区别。
参考:
1。“Isoschizomer。”概述|ScienceDirect主题。
2.“”限制酶:类型和示例。”《学习》,2018年9月4日。
图片提供:
1。“太极” Inks002在英语Wikipedia上(CC BY-SA 3.0)通过Commons Wikimedia
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