单光束和双光束分光光度计之间的差异

Thekey differencebetween single beam and double beam spectrophotometer is thatin single beam spectrophotometer, all the light waves pass through the sample whereas, in double beam spectrophotometer, the light beam splits into two parts and only one part passes through the sample.Spectrophotometersare analytical instruments used to quantify the analytes in a given sample using a light beam. Therefore, this technique measures the absorption of light by the sample.

CONTENTS

1.Overview and Key Difference
2.What is Single Beam Spectrophotometer
3.什么是双光束分光光度计
4.Side by Side Comparison – Single Beam vs Double Beam Spectrophotometer in Tabular Form
5.概括

What is Single Beam Spectrophotometer?

单身的beam spectrophotometer is an analytical instrument in which all the light waves coming from the light source passes through the sample. Therefore, the measurements are taken as the intensity of light before and after the light pass through the sample. These single beam spectrophotometers are more compact and optically simpler than double beam spectrophotometers. And also these instruments are less expensive.

光束通过样品后检测的敏感性很高，因为它使用了非切割光束（因此，存在高能量）。在可见和紫外线波长范围内分析中可以使用单光束分光光度计。

A single beam spectrophotometer measures the concentration of an analyte in a sample by measuring the amount of light absorbed by that analyte. Here, the Beer Lambert Law comes into operation. This law states that the concentration of an analyte is directly proportional to the absorbance.

什么是双光束分光光度计?

Double beam spectrophotometer is an analytical instrument in which the light beam coming from the light source splits into two fractions. One fraction acts as the reference (the reference beam) while the other fraction passes through the sample (sample beam). As a result, the reference beam does not pass through the sample.

样品梁可以测量样品的吸光度。参考光束可以测量吸收（可以将样品梁与参考光束进行比较）。因此，吸收是样品梁（通过样品后）和参考光束之间的比率。分光光度计具有单色仪，该单色器可以从光束上分离所需的波长。参考光束和样品梁重新组合，然后移动到单色仪。因此，这避免或补偿了对样品和参考光束的电子和机械影响。

What is the Difference Between Single Beam and Double Beam Spectrophotometer?

单光束与双光束分光光度计
单身的beam spectrophotometer is an analytical instrument in which all the light waves coming from the light source passes through the sample.	Double beam spectrophotometer is an analytical instrument in which the light beam coming from the light source splits into two fractions.
Light Beam
单身的beam spectrophotometer uses a non-split light beam.	Double beam spectrophotometer uses a light beam that is split into two fractions before passing through the sample.
Measurement
The measurements taken from single beam spectrophotometers are less reproducible because a single light beam is used.	从双光束分光光度计中采取的测量值高度可重复，因为对样品和参考光束的电子和机械影响相等。

概括–单身的Beamvs Double Beam Spectrophotometer

A spectrophotometer is an instrument that analyzes the components of a solution by observing the capability to absorb light. There are two main types of spectrophotometers; single beam and double beam spectrophotometer. The difference between single beam and double beam spectrophotometer is that, in single beam spectrophotometer, all the light waves pass through the sample whereas, in double beam spectrophotometer, the light beam splits into two parts and only one part passes through the sample.

Reference:

1.“单光束分光光度计”。生物组合。Available here
2. “Comparison between Single Beam and Double Beam Atomic Absorption Spectrometer Systems.” Lab-Training.com, 22 July 2015.Available here

Image Courtesy:

1.’spetrophotomer-en’by gyassinemrabettalk-自己的作品，(CC BY-SA 4.0)viaCommons Wikimedia
2.’Simplified UV-vis diagram’ By Sobarwiki- Own work, (Public Domain) viaCommons Wikimedia