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什么是Difference Between Cisgenesis and Transgenesis

September 16, 2021Posted byDr.Samanthi

Thekey difference在偶联和转基因之间is that cisgenesis is thegenetic modificationof a recipient plant with a natural gene from a sexually compatible plant, while transgenesis is the genetic modification in a recipient plant with genes from any non-plant organism or from a donor plant that is sexually incompatible with the recipient plant.

基因修饰的生物具有使用DNA的使用genetic engineering技术。它可以是动物,植物或微生物。进行遗传修饰是为了转移和表达从捐助者到接受者的理想性状。结合和转基因是使用相同的遗传修饰技术进行的两种类型的遗传修饰。合格是指从生物体本身或性兼容的近亲进行的基因进行的遗传修饰。转基因是指来自外来物种的基因所做的遗传修饰,这些基因既不是受体物种也不是亲密的性相对相对。

内容

1.Overview and Key Difference
2.What is Cisgenesis
3.What is Transgenesis
4.相似性 - 屈肌和转基因
5.Cisgenesis vs Transgenesis in Tabular Form
6。摘要 - 结牙与转基因

什么是感染性?

Cisgenesis refers to the genetic modification of a plant with native or natural genes from the plant itself or from a crossable or sexually compatible plant. A desirable gene from one variety of tomato can be transferred into another tomato variety using cisgenesis. The genes are known as cisgenes. Therefore, genetic modification happens in a recipient from a sexually compatible donor.

Cisgenesis and Transgenesis - Side by Side Comparison

图1:Cisgenic土豆

Cisgenetic plants are very much similar to traditionally bred plants. As a result of cisgenesis, the long reproductive cycles of species can be accelerated by transferring favourable genes. Unlike conventional crosses, cisgenesis is fast and more efficient. Cisgenesis does not change the regulatory elements of the gene. The gene has its native promoter, introns, and terminator. As a result of cisgenesis, new traits are instilled in the recipient plant without the use of foreign genes. Hence, cisgenesis is a safe method as traditional breeding. Hence, there is no negative impact on the environment and human health. The development of a potato variety resistant to potato blight is one of the representative applications of cisgenesis.

什么是转基因?

转基因是一种用于将一个或多个基因从一种生物体转移到另一个外国生物体的方法,以使受体具有理想的供体特征。供体是一种性不相容的生物。在转基因中,接受者从外来物种中接收基因,这些基因既不是接受者物种也不是亲密的性相对相对。这样,可以将理想的特征引入生物体中。转基因的目的是在接受者生物体中表达新的特性或特征。

Cisgenesis vs Transgenesis in Tabular Form

Figure 02: Transgenesis

靶基因应首先从供体的DNA中分离出来。限制酶are used to separate out the target gene from the rest of the donor DNA. The target gene should be later extracted out through gel electrophoresis and can be identified using a specific DNA probe. Finally, a vector such as a bacterial plasmid is used to transfer the target gene into the recipient organism. Human insulin is a well-known product of transgenesis.金米is another example of a transgenic organism. However, transgenesis has some unintended consequences and implications on transgenic plants or animals, such as non-target effects of transgenic protein that cause some side effects.

结合和转基因之间有什么相似之处?

  • 转基因和结合是在生物体中进行的两种类型的遗传修饰。
  • 在这两个过程中,接收者都可以接收一个或多个基因。
  • Both processes use similar genetic modification techniques.
  • 因此,它们是人工方法。

什么是Difference Between Cisgenesis and Transgenesis?

Cisgenesis is a genetic modification done using the genes from the organism itself or from a sexually compatible organism, while transgenesis is the genetic modification done using the genes from a sexually incompatible organism. So, this is the key difference between cisgenesis and transgenesis. Moreover, cisgenesis does not cause negative effects on the environment and humans, whereas transgenesis can cause negative effects on humans and the environment.

The following infographic lists the differences between cisgenesis and transgenesis in tabular form for side by side comparison.

摘要 - 结牙与转基因

结菌和转基因是修改生物体遗传物质的两种技术。两种技术都使用基因工程技术。使用来自生物体本身或性兼容供体的基因进行结也。相比之下,转基因是使用性非传染生物的基因进行的。与转基因相比,屈肌更安全。因此,这总结了结合和转基因之间的差异。

Reference:

1. Schouten, Henk J, et al. “Cisgenic Plants Are Similar to Traditionally Bred Plants: International Regulations for Genetically Modified Organisms Should Be Altered to Exempt Cisgenesis.” EMBO Reports, U.S. National Library of Medicine, Aug. 2006.
2. “Transgene.” Wikipedia, Wikimedia Foundation, 18 July 2021.

Image Courtesy:

1.“”方形丙托托斯” By Ronald Hutten, Laboratory of Plant breeding, Wageningen University – Dr. Henk Schouten,(CC BY 3.0)via Commons Wikimedia
2. “Breeding transgenesis cisgenesis” By Smartse at English Wikipedia – Own work(CC BY-SA 3.0)via Commons Wikimedia

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Filed Under:Genetics

关于the Author:Dr.Samanthi

Samanthi Udayangani博士拥有B.Sc.植物科学学位,硕士在分子和应用微生物学和应用微生物学中的博士学位。她的研究兴趣包括生物肥料,植物 - 微生物相互作用,分子微生物学,土壤真菌和真菌生态学。

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