生物负担和内毒素有什么区别

The生物负担和内毒素之间的关键区别is that bioburden is the number of microorganisms found in a given amount of material before undergoingsterilization, while endotoxin is a type of toxin released by microorganisms to the surrounding environment.

微生物测试或分析通常涵盖使用生物学，生化或化学方法检测和枚举微生物的使用。它通常用于检测引起疾病的微生物。微生物测试对于全世界的许多行业，个人和看护人都非常重要。微生物测试对于识别威胁，例如E.coli,葡萄球菌,假单胞菌,Candida,Aspergillus, etc. It also helps to maintain high quality in industries. Bioburden and endotoxin are two important factors in microbial testing in order to detect the level of microbial contamination and to decrease the risk of infection.

内容

1.Overview and Key Difference
2.What is Bioburden
3.What is Endotoxin
4.相似之处 - 生物负担和内毒素
5.表格形式的生物费与内毒素
6。Summary – Bioburden vs Endotoxin

What is Bioburden?

Bioburden is the concentration or quantity of microorganisms found from a given amount of material before conducting a sterilization procedure. This term is often used when performing bioburden testing (microbial limit testing). Bioburden testing is usually performed on pharmaceutical products and medical products for the purpose of quality control. The aim of bioburden testing is to measure the total number of viable microorganisms on a pharmaceutical product or a medical device prior to its final sterilization before use.

Bioburden testing for medical devices made is governed by ISO11737 worldwide. Furthermore, the United States Pharmacopeia (USP) outlines several tests that can be performed to determine bioburden quantitatively on non-sterile drug products. When conducting these tests, it is highly important that they do not either introduce microbes into the test sample or kill microbes in the test sample. Popular bioburden testing methods for samples of drugs include the membrane filtration method and plate count method. Bioburden quantification is normally expressed in the colony-forming unit (CFU). Moreover, bioburden is also associated with biofouling. In the healthcare system, this increases the risk of healthcare-associated infections. Natural convection or liquid cooling in critical care units, clean rooms, and HEPA filters are really helpful in hospital settings to reduce the bioburden.

什么是内毒素？

Endotoxin is a type of toxin released by microorganisms to the surrounding environment. Endotoxins can be deadly to humans. It usually comes from the cell wall components ofgram-negative bacteria. Endotoxins contain both fat components as well as complex sugar components. Therefore, endotoxin is also known as lipopolysaccharide in the scientific literature.

Endotoxins are pyrogens. Pyrogens are fever-inducing substances from microorganisms. When present in sufficient quantity, it can lead to severe signs of inflammation, shock, multiorgan failure and even deaths in humans. Bacterial endotoxin testing can measure the presence and quantity of endotoxins in a specific sample.

生物负担和内毒素之间有什么相似之处？

• Bioburden and endotoxin are two important factors in microbial testing.
• These may contain antigens such as O antigens.
• Both help to detect the level of microbial contamination and to decrease the risk of infection.
• 它们可以toxic and trigger inflammatory reactions in humans.

生物负担和内毒素有什么区别？

Bioburden is the number of microorganisms found in a given amount of material before undergoing sterilization, while endotoxin is a type of toxin released by microorganisms to the surrounding environment. Thus, this is the key difference between bioburden and endotoxin. Bioburden is measured through bioburden testing, while endotoxin is measured through endotoxin testing.

以下信息图列出了与表格形式的生物负荷和内毒素之间的差异，以与并排比较。

Summary – Bioburden vs Endotoxin

Bioburden and endotoxin are two important factors in microbial testing in order to detect the level of microbial contamination. They help to decrease the risk of infection. Bioburden is the number of microorganisms found in a given amount of material before undergoing sterilization procedure, while endotoxin is a type of toxin released by microorganisms to the surrounding environment. So, this is the key difference between bioburden and endotoxin.

Reference:

1.“”Bioburden”。概述| ScienceDirect主题。
2. “Endotoxin”。概述| ScienceDirect主题。

Image Courtesy:

1.“”大板计数分析”埃斯特维兹（Estevezj） - 自己的作品(CC BY-SA 3.0)通过Commons Wikimedia
2. “LPS” By Mike Jones – Own work(CC BY-SA 3.0)通过Commons Wikimedia