这关键区别between flow cytometry and immunohistochemistry is that流式细胞仪是一种利用激光束检测和测量细胞或颗粒群体的物理和化学特征的技术,而免疫组织化学是一种使用的技术monoclonal and polyclonal antibodies检测特定抗原在组织中。
流式细胞仪和免疫组织化学是两种用于诊断疾病的技术,尤其是癌症。流式细胞仪利用激光束来表征细胞的物理和化学特性。免疫组织化学利用单克隆和多克隆抗体检测组织切片中特定抗原。
CONTENTS
1。概述和关键差异
2。What is Flow Cytometry
3。什么是免疫组织化学
4。流式细胞术和免疫组织化学之间的相似性
5。并排比较 - 流式细胞术与表格形式的免疫组织化学
6.Summary
What is Flow Cytometry?
流式细胞仪是细胞生物学中的一种流行技术。该技术检测并测量细胞群体的物理和化学特征。流式细胞仪广泛用于免疫学,分子生物学,细菌学,病毒学,癌症生物学和传染病监测领域。它利用激光作为流体混合物中计数,排序和轮廓单元的光源。因此,它提供了溶液中细胞的快速多参数分析。
This technique begins with injecting a cell sample into the flow cytometer. Flow cytometer has three core systems: fluidics (a flow cell), optics (various filters, light detectors, and light source), and electronics (flow cytometer instrumentation). Prior to that, the sample should be treated with specific dyes depending on the cells being analyzed. Therefore, it utilizes a variety of fluorescent reagents such as fluorescently conjugated antibodies, DNA binding dyes, viability dyes, ion indicator dyes and fluorescent expression proteins, etc. This instrument allows the flow of one cell at a time through the laser beam. When light scatters through the cell and its components, it emits bands of different wavelengths. In this way, tens of thousands of cells can be quickly examined, and the data gathered are processed by a computer.
什么是免疫组织化学?
Immunohistochemistry is a technique that utilizes monoclonal and polyclonal antibodies to determine the tissue distribution of an antigen of interest. It is a common technique in histopathology. It is a microscopy-based, immunostaining technique. This technique facilitates the selective identification and localization of antigens in cells of a tissue based on the specific binding with fluorescently labelled antibodies. This technique is widely used for the diagnosis of cancers since specific tumor antigens are expressed de novo or up-regulated in certain cancers. This technique is very useful in predicting therapeutic response in two important tumors, i.e. carcinoma of the breast and prostate. In addition to disease diagnosis, immunohistochemistry is used in the fields of drug development and biological research. Moreover, immunohistochemistry is useful when detecting and confirming infectious agents in tissues.
该技术需要活检,并将它们与小型集体进行处理,然后将部分与适当的抗体一起孵育。在光或荧光显微镜下可视化抗原抗体的结合位点。
流式细胞术和免疫组织化学之间有什么相似之处?
- 流式细胞仪和免疫组织化学是两种使用荧光标记抗体的技术。
- Both techniques can detect antigens on the cell surface or within cells.
- 因此,两种技术都允许检测疾病,特别是传染病和癌症。
流式细胞仪和免疫组织化学有什么区别?
流式细胞仪是一种基于激光的技术,可检测和测量细胞群体的物理和化学特征。免疫组织化学是一种基于显微镜的技术,可在组织细胞中选择性鉴定和定位抗原。因此,这是流式细胞仪和免疫组织化学之间的关键区别。此外,流式细胞仪使用激光束,而免疫组织化学需要抗体。流式细胞仪是流式细胞仪中所需的主要仪器,而免疫组织化学需要光或荧光显微镜。
以下是流式细胞术和免疫组织化学之间的差异的摘要。
摘要 - 流式细胞术与免疫组织化学
流式细胞术需要流式细胞分析仪,immunohistochemistry needs an ordinary or fluorescent microscope. Moreover, flow cytometry utilizes a laser beam, while immunohistochemistry utilizes monoclonal and polyclonal antibodies. In addition, the cost of immunohistochemistry is relatively low compared to flow cytometry. Thus, this is the summary of the difference between flow cytometry and immunohistochemistry. Both techniques are useful in diagnosing diseases such as cancers and infectious diseases.
参考:
1. McKinnon,Katherine M.“流式细胞仪:概述。”美国国家医学图书馆,当前的免疫学方案,2018年2月21日,在这里可用。
2. Duraiyan,Jeyapradha等。“免疫组织化学的应用。”药学与生物学杂志,MED KNowning Publications&Media Pvt Ltd,2012年8月,在这里可用。
图片提供:
1. Biol的“ FACS-Buisje” - 通过自己的作品(公共领域)通过下议院维基梅迪亚
2. Nakos组织病理学的“乳房免疫组织化学” - 自己的工作(CC BY-SA 4.0)通过下议院维基梅迪亚
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