Thekey difference between IR and UV and visible spectroscopyIR光谱法使用光谱的低能红外部分,而紫外线和可见光谱法使用了紫外线和可见区域electromagnetic spectrum.
There are different spectroscopic techniques according to the波长range that is being measured. IR and UV and visible spectroscopy are two such spectroscopic techniques.
内容
1.Overview and Key Difference
2.是什么IR Spectroscopy
3.什么是紫外线和可见光谱
4.IR与紫外线和可见光谱以表格形式
5.摘要 - IR vs UV与可见光谱
是什么IR Spectroscopy?
IR spectroscopy or红外光谱法(也称为振动光谱法) is the measurement of the interaction of IR radiation with the matter by absorption, emission, or reflection. This method is useful in studying and identifying chemical substances or functional groups in solid, liquid, or gaseous forms. Moreover, we can use IR spectroscopy to characterize new materials and identify and verify known and unknown samples.
IR spectroscopy involves the absorption frequencies by molecules that are characteristic of the structure. Typically, these absorptions occur at resonant frequencies (it is the frequency of the absorbed radiation that matches the vibrational frequency). Particularly, in the Born-Oppenheimer and harmonic approximations, resonant frequencies are associated with the normal modes of vibration that correspond to the molecular electronic ground state potential energy surface. Moreover, resonant frequencies are related to the strength of the bond and the mass of the atoms at each end. Therefore, the frequency of these vibrations is associated with a particularly normal mode of motion and a particular bond type.
什么是紫外线和可见光谱?
紫外和可见光谱或UV-VIS光谱法is an analytical instrument that analyzes liquid samples by measuring its capability to absorb radiation in ultraviolet and visible spectral regions. This means this absorption spectroscopic technique uses light waves in visible and adjacent regions in the electromagnetic spectrum. Absorption spectroscopy deals with the excitation of electrons (movement of an electron from the ground state to the excited state) when the atoms in a sample absorb光能.
电子激发发生在包含PI电子或非键值电子的分子中。如果样品中分子的电子很容易受到激发,则样品可以吸收更长的波长。结果,PI键或非键轨道中的电子可以从紫外线或可见范围内的光波中吸收能量。
The major advantages of UV-Visible spectrophotometer include simple operation, high reproducibility, cost-effective analysis, etc. In addition, it can use a wide range of wavelengths to measure analytes. The basic components of UV-visible spectroscopy include a light source, a sample holder, diffraction gratings in the monochromator, and a detector.
紫外可见分光光度计可用于量化溶液中的溶质。该仪器可用于量化分析物,例如过渡金属和共轭有机化合物(含有交替PI键的分子)。我们可以使用该工具来研究解决方案,但有时科学家也使用该技术来分析固体和气体。
IR和UV和可见光谱之间有什么区别?
Spectroscopy is the study of the absorption and emission of light and other radiation by matter. There are different types, such as IR spectroscopy and UV-visible spectroscopy. The key difference between IR and UV and visible spectroscopy is that IR spectroscopy uses the low-energy infrared part of the spectrum, whereas UV and visible spectroscopy use UV and visible regions of the electromagnetic spectrum.
Below is a summary of the difference between IR and UV and visible spectroscopy in tabular form.
摘要 - IR和UV与可见光谱
Spectroscopy is an important analytical technique useful in studying different chemical substances. IR spectroscopy and UV-visible spectroscopy are two types of this analytical technique. The key difference between IR and UV and visible spectroscopy is that IR spectroscopy uses the low-energy infrared part of the spectrum, whereas UV and visible spectroscopy use UV and visible regions of the electromagnetic spectrum.
Reference:
1.“”了解光谱法和光谱法。”ATA Scientific.
Image Courtesy:
1.“”Portable Screening Devices (1435) (8225044148)“ 经过The U.S. Food and Drug Administration – (Public Domain) via Commons Wikimedia
2. “GENESYS™ 10S UV-Vis Spectrophotometer – Thermo Fisher Scientific“ 经过PhotoLanda(CC BY-NC-SA 2.0)通过Flickr
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