链接器和适配器之间的区别

Thekey differencebetween linker and adaptor is thata linker does not have cohesive ends while an adaptor has one cohesive end.

脱氧核糖核酸ligation is the process of joining two脱氧核糖核酸molecules together, forming phosphodiester bonds. The enzyme called DNA ligase catalyzes this reaction. It is one of the critical steps in modern molecular biological fields such as重组DNA技术和DNA克隆。连接效率取决于要连接的DNA分子的末端。DNA末端有两种类型的粘性末端和钝端。连接效率高，粘性端高于钝端。如果靶DNA分子具有钝器末端，则称为衔接子或接头的分子将有用。适配器和接头是化学合成的oligonucleotide有助于DNA连接的分子。他们也有内部限制站点。适配器具有一个粘性端和一个钝端，而Linker具有两个钝器。

CONTENTS

1.Overview and Key Difference
2.What is a Linker
3.What is an Adaptor
4.Similarities Between Linker and Adaptor
5.Side by Side Comparison – Linker vs Adaptor in Tabular Form
6.概括

什么是链接器？

Linker is a chemically synthesized oligonucleotide sequence that is double-stranded. Linker has two blunt ends. Linker is used to ligate DNA molecules that have blunt ends to vectors. It contains one or more internal restriction sites. These restriction sites work as recognition sites for restriction enzymes.

After ligation, DNA is restricted again with restriction enzymes to produce cohesive ends. EcoRI-linkers and sal-I linkers are commonly used linkers.

What is an Adaptor?

An Adaptor is a double-stranded oligonucleotide sequence used to link two DNA molecules together. It is a short sequence with one blunt end and one sticky or cohesive end. Therefore, it consists of a single-stranded tail at one end, which enhances the efficiency of DNA ligation.

Moreover, the adaptor has internal restriction sites. Therefore, after ligation, DNA can be restricted with appropriate restriction enzymes in order to create a new protruding terminus. One disadvantage of adaptors is that two adaptors can form dimmers by base pairing with themselves. This can be avoided by treating them with the enzyme called alkaline phosphatase.

链接器和适配器之间有什么相似之处？

• Both linker and adaptor are double-stranded short oligonucleotide sequences.
• 他们带着我nternal restriction sites.
• Moreover, they are chemically synthesized DNA molecules and are synthetic molecules.
• They can link two DNA molecules together.
• 连接连接器和适配器的连接后，DNA再次受到限制酶的限制，以产生粘性末端。

What is the Difference Between Linker and Adaptor?

接头是一种化学合成的短寡核苷酸双链，具有两个钝端。适配器是一种化学合成的短寡核苷酸双工，具有一个粘性端和一个钝端。因此，这是链接器和适配器之间的关键区别。此外，适配器可以形成二聚体，而链接器不会形成二聚体。因此，这是链接器和适配器之间的另一个重要区别。

Below is a summary of the differences between linker and adaptor in tabular form.

概括– Linker vs Adaptor

Linker and adaptor are two types of chemically synthesized oligonucleotides that are useful in ligating blunt-end DNA. Linker has two blunt ends, while adaptor has one blunt end and one cohesive end. So, this is the key difference between linker and adaptor. They are double-stranded molecules that have internal restriction sites. They are widely used in recombinant DNA technology and DNA cloning.

Reference:

1. “Linker DNA.” Wikipedia, Wikimedia Foundation, 31 Aug. 2020,Available here.
2. Rothstein, R.J., et al. “[7] Synthetic Adaptors for Cloning DNA.” Methods in Enzymology, Academic Press, 7 Jan. 2004,Available here.

Image Courtesy:

1. Karahavet的“ Bless Workflow（更新V2）” - 自己的工作(CC BY-SA 4.0)viaCommons Wikimedia
2. “Library preparation for the SOLiD platform” By Philippe Hupé – Emmanuel Barillot, Laurence Calzone, Philippe Hupé, Jean-Philippe Vert, Andrei Zinovyev, Computational Systems Biology of Cancer Chapman & Hall/CRC Mathematical & Computational Biology, 2012(CC BY-SA 3.0)viaCommons Wikimedia