T4 DNA连接酶和E大肠杆菌DNA连接酶有什么区别

Thekey difference between T4 DNA ligase and E. coli DNA ligase是T4 DNA连接酶是一种从中分离出来的酶bacteriophageT4 while E. coli DNA ligase is an enzyme that is isolated from the bacteriumE. coli。

DNA ligase is a specific type of enzyme that facilitates the joining of DNA strands together through the formation of a磷酸酯键。It plays an important role by repairing single-strand breaks and double-strand breaks in the DNA of living organisms. Moreover, DNA ligase is used in both DNA repair andDNA replication。除上述用途外，DNA连接酶还广泛用于分子生物学实验，例如重组DNA技术。T4 DNA连接酶和E. coliDNA ligase are two types of DNA ligase enzymes.

CONTENTS

1.Overview and Key Difference
2.What is T4 DNA Ligase
3.什么是大肠杆菌DNA连接酶
4.Similarities – T4 DNA Ligase and E Coli DNA Ligase
5.T4 DNA Ligase vs E Coli DNA Ligase in Tabular Form
6.Summary – T4 DNA Ligase vs E Coli DNA Ligase

What is T4 DNA Ligase?

T4 ligase is a DNA joining enzyme that is isolated from bacteriophage T4. T4 bacteriophage is a virus that infectsEscherichia coli。T4连接酶是分子生物实验室研究中最常用的DNA连接酶。通常，T4连接酶催化了5'磷酸盐和3'邻近核苷酸的3'羟基之间的两条粘性或钝性链的连接。它还可以催化RNA的连接到双链分子中的DNA或RNA链。但是，该酶不会连接单链核酸。此外，它还可以将钝性的DNA结合起来，效率要高得多E. coliDNA ligase.

该酶需要ATP作为辅因子。与野生型T4 DNA连接酶相比，一些基因工程研究实验已经产生了更多的活性DNA连接酶。在其中一种方法中，它们通过将T4 DNA连接酶梳为P50或NF-KB分子来创建融合酶分子。与野生型T4 DNA连接酶相比，这种新的T4连接酶在钝性结膜中的活性高160％。此外，T4 DNA连接酶的最佳温度更精确地催化连接反应是16°C。

什么是大肠杆菌DNA连接酶?

E. coli DNA ligase is an enzyme that is isolated fromE. coli细菌。负责制造的基因E. coliDNA ligase is lig gene. TheE. coliDNA, ligase as well as most prokaryotes DNA ligases, use energy that is gained by NAD (nicotinamide adenine dinucleotide) to create the phosphodiester bond.

E. coliDNA连接酶连接酶,可以加入DNAfragments by catalyzing the formation of phosphodiester bonds between double-stranded DNA fragments containing 5’ phosphate termini and 3’ hydroxyl termini. Moreover, it does not ligate blunt-ended DNA except under special conditions such as molecular crowding with polyethylene glycol. It also cannot join RNA to DNA efficiently. Furthermore, the activity ofE. coliDNA ligase can be enhanced by the presence of DNA polymerase 1 at the right concentration. However, when the concentration of DNA polymerase 1 enzyme is higher, it has adverse effects onE. coliDNA ligase activity.

What are the Similarities Between T4 DNA Ligase and E Coli DNA Ligase?

• T4 DNA连接酶和E-coliDNA ligase are two types of DNA ligase enzymes.
• Both are proteins made up of amino acids.
• These enzymes catalyze the formation of a phosphodiester bond between juxtaposed 5’ phosphate and 3’ hydroxyl termini in duplex DNA.
• They have extensive uses in molecular biology experiments such as recombinant DNA technology.

T4 DNA连接酶和E大肠杆菌DNA连接酶有什么区别?

T4 DNA ligase is an enzyme that is isolated from bacteriophage T4 whileE. coliDNA ligase is an enzyme that is isolated fromE. coli细菌。Thus, this is the key difference between T4 DNA ligase andE. coliDNA ligase. Furthermore, the source of energy for T4 DNA ligase is ATP. On the other hand, the source of energy forE. coliDNA ligase is NAD.

The below infographic presents the differences between T4 DNA ligase andE coliDNA ligase in tabular form for side by side comparison.

Summary – T4 DNA Ligase vs E Coli DNA Ligase

T4 DNA连接酶和E. coliDNA ligase are two enzymes that catalyze the formation of a phosphodiester bond between juxtaposed 5’ phosphate and 3’ hydroxyl termini in duplex DNA. T4 ligase is an enzyme that is isolated from bacteriophage T4 whileE. coliDNA ligase is an enzyme that is isolated fromE. coli细菌。So, this is the key difference between T4 DNA ligase andE coliDNA ligase.

Reference:

1. “T4 DNA Ligase。”新英格兰Biolabs：生命科学行业的试剂。
2. “E. Coli DNA Ligase。”新英格兰Biolabs：生命科学行业的试剂。

Image Courtesy:

1. “Site directed mutatgenesis” By Mjeltsch – Own work(CC BY-SA 4.0)via Commons Wikimedia
2. “DNA mismatch repair” Kenji Fukui -NCBI(CC BY 3.0)via Commons Wikimedia