What is the Difference Between Agarose and Polyacrylamide Gel Electrophoresis

Thekey difference琼脂糖和聚丙烯酰胺凝胶电泳之间是琼脂糖凝胶电泳使用水平倒入的琼脂糖凝胶将相对较大的DNA片段分离，而聚丙烯酰胺凝胶电泳则使用垂直倒入的聚丙烯酰胺凝胶来分离更短暂的聚丙烯酰胺凝胶nucleic acidfragments.

Electrophoresis is a type of technique that uses an electric field applied across a gel matrix to separate biomolecules like DNA, RNA, and proteins. This separation of biomolecules such asDNA, RNA，通过电泳的蛋白质基于电荷和大小。样品被加载到凝胶的孔中。后来，电场在整个凝胶上施加。该场导致负电荷分子向正电极移动。凝胶基质充当分子筛，最小的分子通过该分子筛，而大分子移动缓慢。这可以根据电荷和大小将分子分离。因此，琼脂糖和聚丙烯酰胺凝胶电泳是两种类型的凝胶电泳技术，主要有助于根据它们的大小和电荷分离分子。

内容

1.Overview and Key Difference
2.什么是琼脂糖凝胶电泳
3.什么是聚丙烯酰胺凝胶电泳
4.Similarities – Agarose and Polyacrylamide Gel Electrophoresis
5.Agarose vs Polyacrylamide Gel Electrophoresis in Tabular Form
6。Summary – Agarose vs Polyacrylamide Gel Electrophoresis

什么是琼脂糖凝胶电泳?

琼脂糖凝胶电泳是一种使用琼脂糖凝胶将生物分子（例如DNA和RNA）分开的技术。这是一种用于主要按大小分离核酸的技术。该技术中使用的称为琼脂糖的主要化合物是多糖. It comes from seaweeds. Agarose can be dissolved in boiling buffer and then can be poured into a tray that is kept horizontally. In the tray, it becomes solidified when it cools down to form a slab. Agarose gels are poured with a comb in place to make wells into which nucleic acids such as DNA or RNA are loaded once the gel has solidified.

凝胶后来浸入适当的缓冲液中，并在整个凝胶上施加电流。DNA具有均匀的负电荷，与分子的序列组成无关。因此，DNA分子将从阴极（ - ）迁移到阳极（+）。迁移速率直接取决于分子的大小。最大的大分子在凝胶上航行最困难的时期。另一方面，较小的大分子迅速且很容易地穿过凝胶。

什么是聚丙烯酰胺凝胶电泳?

Polyacrylamide gel electrophoresis (PAGE) is a technique that uses polyacrylamide gels to separate out biomolecules. It is a technique that is widely used to separate biological macromolecules, usually proteins and nucleic acids, according to their electrophoretic mobility. The hydration of acrylonitrile results in the formation of acrylamide molecules by nitrile hydratase. Acrylamide is soluble in water, and upon the addition of free radical initiators, acrylamide polymerizes, resulting in the formation of polyacrylamide gel. Normally, increased concentrations of acrylamide result in decreased pore size in the gel. Polyacrylamide gels are poured vertically, unlike agarose gels.

In polyacrylamide gel electrophoresis, the molecules may run in their native state, preserving the higher-order structure of molecules. This method is callednative PAGE. Alternatively, a chemical denaturant can also be added to remove the higher-order structure and turn the molecule into an unstructured molecule whose mobility depends only on its length. This type is calledSDS-PAGE.

琼脂糖和聚丙烯酰胺凝胶电泳有什么相似之处？

• Agarose and polyacrylamide gel electrophoresis are two types of gel electrophoresis techniques.
• 实际上，它们是分子生物学技术。
• Both techniques are used to detect biological macromolecules like DNA and proteins.
• 这些技术是由熟练的技术人员或研究人员完成的。
• 在这两种技术中，大分子的分离都是基于电荷和大小的。
• Both techniques allow the separation of nucleic acids like DNA and RNA.

What is the Difference Between Agarose and Polyacrylamide Gel Electrophoresis?

Agarose gel electrophoresis is a technique that uses horizontally poured agarose gels to separate out biomolecules, while polyacrylamide gel electrophoresis is a technique that uses vertically poured polyacrylamide gels to separate out biomolecules. This is the key difference between agarose and polyacrylamide gel electrophoresis. Furthermore, agarose gel electrophoresis is used for the separation of DNA and RNA, while polyacrylamide gel electrophoresis is used for the separation of nucleic acids such as DNA, RNA, or proteins.

The following table summarizes the difference between agarose and polyacrylamide gel electrophoresis.

Summary – Agarose vs Polyacrylamide Gel Electrophoresis

Agarose and polyacrylamide gel electrophoresis are two types of gel electrophoresis techniques used in molecular biology laboratories. Agarose gel electrophoresis uses an agarose gel to separate out biomolecules. Agarose is generally not toxic to humans, while polyacrylamide is toxic to humans. Moreover, agarose gel electrophoresis shows a low resolution while polyacrylamide gel electrophoresis shows a more resolution. Polyacrylamide gel electrophoresis uses a polyacrylamide gel to separate out biomolecules. This summarizes the difference between agarose and polyacrylamide gel electrophoresis

Reference:

1.“”琼脂糖凝胶电泳”。概述|科学irect Topics.
2. “Polyacrylamide Gel Electrophoresis”。概述|科学irect Topics.

Image Courtesy:

1.“琼脂糖凝胶电泳”PlaxcoLab（CC由2.0）via Flickr
2. “Polyacrylamide gel electrophoresis of Proteins” By Jean-Etienne Minh-Duy Poirrier from Bruxelles, Belgium – Proteins in a 1D gel electrophoresis(CC BY-SA 2.0)via Commons Wikimedia