Thekey difference倾注平皿和扩散板之间a known volume of the sample is spread on the surface of the琼脂中等片中的片板,而在倒板中,将已知的样品与琼脂混合,然后倒入板中。在比较这两种技术的准确性时,浇注板的精度比扩散板更高。
标准板计数方法是一种基于生长的方法,可计算样品中生存(可生长/可栽培/可行)微生物的数量。在许多微生物领域中,它是一种强大的使用方法,用于分析生物微生物的数量。食品和乳制品,医学,环境,水生和农业,微生物遗传学,分子微生物学,生长培养基开发和生物技术(生物反应器技术,发酵,废物/废水处理等)等领域都利用了这项技术。此外,执行标准板计数的主要方法有两种:它们是传播板技术和倒入板技术。
内容
1.Overview and Key Difference
2.What is Pour Plate
3.What is Spread Plate
4.Similarities Between Pour Plate and Spread Plate
5.Side by Side Comparison – Pour Plate vs Spread Plate in Tabular Form
6.概括
What is Pour Plate?
Pour plate technique is a microbial method to enumerate some viable cells present in a sample. The speciality of the pour plate method is that a known volume of the sample is first mixed with agar and then poured into the plate.
Other steps are similar to the spread plate technique discussed in the next section. The next step after pouring the agar mixed with the sample is to allow it to solidify and incubate. After incubation, counting the number of viable colonies is essential to calculate the final CFU for 1 g or 1 ml.
What is Spread Plate?
扩散板是一种在样品中列举可行细胞的技术。对于此技术,样品必须进行连续稀释,以确保至少产生可数量的菌落。这里的过程是将一组稀释量放在琼脂板的表面上,并用玻璃撒布机快速,均匀地将其散布在琼脂表面上,玻璃撒布机燃烧和冷却。进行重复是为了获得可靠的平均值所必需的。下一步是在短时间内干燥这些板,然后将其倒置并在适当的温度下放置在孵化器中,以进行生长和设定的孵化时间。
孵育后,检查板将显示出增长。在一种或多个稀释液中,接种剂中存在的细胞数量(例如100或200μl)将在琼脂表面上产生30至300个离散菌落。少于30个殖民地计数在统计上是不可靠的。超过300以上的计数很难枚举,容易出错。
What are the Similarities Between Pour Plate and Spread Plate?
- Spread plate and pour plate are two techniques to obtain number of viable cells present in a sample.
- Both are straight forward methods.
- Sampling errors can happen in both methods.
- Limitations of growth conditions affect the outcome of both methods.
- Technical errors are also can interfere the final result of both methods.
- Though viable, the two methods are not in use to grow un-culturable bacteria.
浇注板和涂抹板有什么区别?
Pour plate is a microbial technique to enumerate a number of viable cells in a sample. Spread plate technique is another technique to enumerate the bacteria grown on the surface of the media. In pour plate technique, the process is to add the sample onto the solidified medium surface on the pour plate. But, in the spred plate technique, the process is to mix the sample with the molten agar and then pour that into the plate.
With regard to the accuracy of these two techniques, pour plate has a higher accuracy than the spread plate. Moreover, unlike in a pour plate, a glass spreader is used to spread the sample evenly on the surface on a spread plate. Further, using pour plate, it is possible to enumerate the aerobes, anaerobes and facultative anaerobes. However, using the spread plate it is only possible to enumerate the aerobes. Furthermore, solidified agar plates are necessary to perform spread plate whereas liquid molten agar media are necessary for pour method.
概括– Pour Plate vs Spread Plate
Pour plate and Spread plate are two techniques in microbiology to facilitate the enumeration of microbial cells in a sample. Both methods are growth based hence, measure the viable cells. During the pour plate, a known volume of the sample is mixed with the molten agar and poured into the plate. During the spread plate, a known volume is spread on the surface of the solidified agar medium. This is the difference between pour plate and spread plate.
Reference:
1.“Making a Pour Plate | Nuffield Foundation.” The Truth behind SEN Statements in Mainstream Primary Schools | Nuffield Foundation.Available here
2.“Microbiology – 004 – Spread Plate Method.” Microbiology – 007 – Carbohydrate Fermentation Test | Microbiology Undergraduate Program.Available here
Image Courtesy:
1.’Ecoli colonies’By Madprime – Own work,(CC BY-SA 3.0)通过Commons Wikimedia
2.’34743904365'By R6,州和私人林业,森林健康p ..(公共领域)通过Flickr
Niraanjan Gohain说
I like it and very easy for learning .Thank you so much .