Key Difference – Positive vs Negative Control
Scientificexperiments始终使用控件执行以获得可靠的结果。从实验中获得的结果可以进行严格的比较,分析和解释。因此,维持控制实验至关重要,应将其包括在实验设计中以增加统计validityof the data set. There are two types of control treatments known as positive control and negative control. Negative and positive controls are defined based on thevariablesor the treatments of the experiment. Positive control is an experimental treatment which results in the desired effect the researcher expects. Negative control is an experimental treatment which does not result in the desired effect of the experimental variable. Thus, the key difference between the positive and negative control is,阳性对照会产生响应或期望的效果,而阴性对照不会产生实验的反应或没有所需的效果。
CONTENTS
1.Overview and Key Difference
2.什么是积极控制
3.What is Negative Control
4.Side by Side Comparison – Positive vs Negative Control
5.概括
What is a Positive Control?
积极的控制是一个experimental control which gives a positive result. It does not have the independent variable that researcher tests. However, it shows the desired effect which is expected from the independent variable. Positive control is a useful proof to show that the protocols, reagents and the equipment are functioning well without any errors. If experimental errors occur, positive control will not produce the correct outcome. Therefore researcher can identify and optimize the procedure without wasting time, effort and the money. For example, when testing a plant extract for antimicrobial properties in antimicrobial compound experiment, a known antimicrobial compound containing solution is used as a positive control. It produces a prominent bacterial growth inhibition zone around the positive control disk as shown in figure 01. If you observed a prominent growth inhibition zone around the disk in the positive control, it says that the experimental setup is working well without errors.
What is a Negative Control?
阴性对照不会对治疗产生反应。在实验中,应以不产生实验结果的方式设计负对照。在上面的示例中,应用无菌蒸馏水浸泡用作阴性对照的纸盘。在无菌蒸馏水中,没有抗菌化合物。因此,细菌可以生长而不会抑制。如果在阴性对照中观察到抑制作用,则表明实验有问题。
What is the difference between Positive and Negative Control?
Positive vs Negative Control |
|
积极的控制是一个experimental treatment which is performed with a known factor to get the desired effect of the treatment. | Negative control is an experimental treatment which does not result in the desired outcome of the experiment. |
Importance | |
Positive control is an important part of an experiment. | Negative control is also an important part of an experiment |
Reliability of the Experiment | |
Positive control increases the reliability of the experiment. | 阴性对照提高了实验的可靠性。 |
概括– Positive vs Negative Control
控件是实验的重要元素。它们在科学实验中维持,以消除实验错误和偏见。对照实验的结果可用于对实验进行验证的统计分析。因此,可以通过对照处理来提高实验的可靠性。有两种类型的对照,即阳性和负面。阳性对照显示治疗的预期作用。阴性对照不会显示治疗的作用。这是阳性对照和阴性对照之间的差异。使用对照的实验称为受控实验。实验的阳性和阴性对照确保实验正确完成,并且实验的结果受自变量的影响。
References:
1. Weinberg, Robert A. “Positive and negative controls on cell growth.” ACS Publications. N.p., 10 Oct. 1989. Web. 04 Apr. 2017
2. Lipsitch, Marc, Eric Tchetgen Tchetgen, and Ted Cohen. “Negative Controls: A Tool for Detecting Confounding and Bias in Observational Studies.” Epidemiology (Cambridge, Mass.). U.S. National Library of Medicine, May 2010. Web. 04 Apr. 2017
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