Key Difference – Metabolomics vs Metabonomics
Metabolitesare the small molecules involved in metabolic reactions in the cells. Metabolites include metabolic intermediates, hormones, secondary metabolites, signaling molecules, etc. They are the functioning molecules of biochemical reactions of the cells. A complete set of metabolites of a biological sample or a single organism is known as metabolome. Metabolome is a dynamic collection which changes every second within the body. Metabolomics and metabonomics are two terms related to the study of metabolome of an organism. The key difference between metabolomics and metabonomics is thatmetabolomics is more concerned about normal endogenous metabolismand metabolic profiling at a cellular or organ levelwhile代谢学更加关注将新陈代谢分析与由于环境因素,疾病,肠道微生物和比较新陈代谢分析等的代谢扰动信息的信息。Metabolomics is mainly driven by mass光谱法, and metabonomics uses NMR spectroscopy for metabolite analysis.
内容
1.Overview and Key Difference
2.What is Metabolomics
3.What is Metabonomics
4.并排比较 - 代谢组学与代谢学
5.概括
What is Metabolomics?
Metabolomics is the study of metabolic processes that occur in细胞, biofluids,tissuesor organisms. It includes the identification and quantification of cellular metabolites using good analytical and statistical tools. Metabolomics is considered as an important study since it reveals information about the metabolism of an organism.
为了研究代谢反应的底物和产物,代谢组学使用质谱法作为一个分析平台。质谱法揭示了代谢物及其浓度的类型,反映了细胞或组织的实际生化状态。因此,代谢组学可以被认为是分子的最佳代表phenotype一个有机体。代谢组学是一种使技术of small molecules of the organisms and compared to other omic studies; metabolomics is very important since it directly reflects the current status of the biochemical reaction of the cells.
Metabolomics can also be considered as an extension of蛋白质组学因为它们是由于其活动而产生的enzymes如下图所示,是蛋白质。
What is Metabonomics?
Metabonomics is defined as a quantitative measurement of multiparametric metabolic responses at specific times in relation to pathophysiologic stresses or genetic modification. This is usually applied to studies carried out about human nutrition and responses to drugs or disease. This approach was pioneered by Jeremy Nicholson at Imperial College, London and it has been used in a number of fields including toxicology, disease diagnosis, nutrition, etc. Historically, metabonomics was one of the first methods to apply the scope of systems biology to studies of metabolism.
代谢学更关心比较生化特征,而不是鉴定代谢组学中的单个代谢化合物。因此,代谢学可以定义为代谢组学的子集。它主要强调不同人群的代谢谱相对于疾病,环境压力,遗传改性,营养,药物等的比较。因为代谢物是疾病状况,药物的作用,环境压力,暴露于毒素的良好标志物, ETC。
替代研究揭示了细胞内和细胞外代谢产物。它使用高吞吐量技术同时又一次地分析了大量的代谢产物,从而可以对细胞的生理状态进行时间研究。它还揭示了代谢物与生物体的病理状况之间的相关性。为了支持代谢研究,基因组学和蛋白质组学可以使用,因为它们相互关联,如上图所示。
代谢组学和代谢学有什么区别?
Metabolomics vs Metabonomics |
|
Metabolomics is the study of the complete set of metabolites of an organism | Metabonomics is the quantitative study of multiparametric metabolic responses of living systems in temporal basis to pathophysiological stimuli or genetic modification. |
Main Concern | |
Metabolomics is more concerned with metabolic profiling and identifying individual metabolites present in the cells. | Metabonomics is more concerned with comparing metabolites or metabolic profiles of populations in relation to genetic modifications, diseases, environmental stresses, pathological stimuli, drugs, etc. |
信息 | |
代谢组学主要集中于内源代谢。 | Metabonomics is not restricted to endogenous metabolism. It extends to find information about perturbations of metabolism caused by internal and external factors such as dietary patterns, toxins, disease processes, etc. |
Analytical Tools | |
It uses mass spectrometry as the prime analytical platform | 替代代表学使用NMR光谱作为主要的分析平台。 |
摘要 - 代谢组学与代谢学
代谢组代表了细胞或生物体中存在的称为代谢产物的完整小分子。代谢组学是整个代谢组生成代谢谱的研究。代谢组学允许科学家测量细胞或生物体的实际生理状态。代谢组学是代谢组学的一部分,它扩展了有关生物系统对病理生理刺激和遗传修饰的多参数代谢反应的信息。代谢学不仅关注像代谢组学这样的新陈代谢分析;它比较了与其他因素有关的代谢谱,例如环境应激,疾病,毒素等。代谢组学和代谢学都涉及对生物体的代谢物进行研究,以测量细胞的实际生理状态。因此,有时这两个被视为同义词,而无需考虑代谢组学和代谢学之间的差异。
Reference:
1. Roessner,Ute和Jairus Bowne。“什么是代谢组学。”生物技术 - 代谢组学的全部内容是什么?N.P.,2009年4月。网络。2017年4月24日
2. Jeremy J. Ramsden (1). “Metabolomics and Metabonomics.” Springer. Springer London, 01 Jan. 1970. Web. 25 Apr. 2017
Image Courtesy:
1. LMAPS(谈话)的“代谢组学模式” - 自己的作品(CC BY-SA 3.0)viaCommons Wikimedia
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