Difference Between Harris and Mayer’s Haematoxylin

Thekey differencebetween Harris and Mayer’s haematoxylin is that哈里斯血久毒素用于regressive stainingwhile Mayer’s haematoxylin is used inprogressive staining。

Haematoxylin is a basic dye that is commonly used in histopathology. It is a natural dye extracted from the treeHaematoxylon campechianum。这是深蓝色或紫色染料。赤道蛋白可以证明细胞和组织成分的微观核细节。因此，这种染料具有正电荷，因此与带负电荷的物质（例如DNA and RNAand stains them in violet colour. Haematoxylin requires a mordant to help the linking with the tissue components. A mordant is a chemical, especially a salt of aluminum, iron, tungsten, which facilitates the linking of a dye to the tissue component.

基于它们包含的媒种，有几种类型的血久毒素可用。其中一些是Ehrlich的，Mayer's，Harris，Gill's，Delafield's，Cole's和Carazzi的血久毒素。Harris和Mayer的赤毒素制剂都是基于铝的源自降血石蛋白。

内容

1.Overview and Key Difference
2.What is Harris Haematoxylin
3.What is Mayer’s Haematoxylin
4.Similarities Between Harris and Mayer’s Haematoxylin
5.Side by Side Comparison – Harris vs Mayer’s Haematoxylin in Tabular Form
6.概括

什么是哈里斯血久毒素？

Harris haematoxylin is a basic dye which is commonly used regressively inhistologylabs for routine H and E staining. Harris haematoxylin uses aluminium as its mordant in order to link with the tissue components. During regressive staining, the tissue is over-stained with Harris haematoxylin. Therefore, it requires differentiation with dilute acid alcohol.

哈里斯血久毒素具有高浓度的血久毒素。因此，它迅速扩散到整个细胞上。哈里斯血久毒素在深紫蓝色中染色核。

What is Mayer’s Haematoxylin?

Mayer’s haematoxylin is a basic dye used in progressive staining. It takes 5 to 10 minutes for staining. Similar to Harris haematoxylin, Mayer’s haematoxylin is an aluminium-based mordant haematoxylin. Therefore, it uses aluminium as its mordant in order to link with the tissue component.

Mayer’s haematoxylin stain has a low concentration of haematoxylin. Hence, it slowly and selectively stains chromatin. It does not require differentiation to remove excess stain. Moreover, Mayer’s haematoxylin solutions are fairly tolerant of minor variations in the application time.

What are the Similarities Between Harris and Mayer’s Haematoxylin?

• Harris and Mayer’s Haematoxylin are two types of haematoxylins classified according to the mordant they contain.
• 它们是从植物中提取的天然染料。
• Both are aluminium-based mordant haematoxylins.
• In fact, they are nuclear stains.
• They colour nuclei in blue.
• 它们通常用于组织学和组织病理学中的常规染色。
• Their stability is high.
• These aluminium based haematoxylins are more practical for extended use.

What is the Difference Between Harris and Mayer’s Haematoxylin?

The key difference between Harris and Mayer’s haematoxylin is their use. Harris haematoxylin is used in regressive staining while Mayer’s haematoxylin is used in progressive staining. Harris haematoxylin has a high concentration of haematoxylin. Therefore, it rapidly diffuses over the entire cell. Mayer’s haematoxylin stain, on the other hand, has a low concentration of haematoxylin. Hence, it slowly and selectively stains chromatin.

下面的信息图表列出了哈里斯和梅耶的血久毒素之间的更多差异。

摘要 - 哈里斯vs梅耶的血久毒素

Haematoxylin is the most popular used dye in histology. Therefore, this stain is commonly used for routine histological examination of tissue components. Harris haematoxylin and Mayer’s haematoxylin are two alum haematoxylin solutions. Both stain nuclei in dark blue colour. Harris haematoxylin is used in regressive staining while Mayer’s haematoxylin is used in progressive staining. Harris haematoxylin rapidly over-stain the tissue, hence requires differentiation to remove excess stain. Mayer’s haemoxylin does not over-stain the tissue. Thus, this summarizes the difference between Harris and Mayer’s haematoxylin.

Reference:

1.”血久毒素 - 蓝调的故事。”vibath，Available here。
2.“常规H＆amp;e”。slideshare.net，2020年，Available here。

Image Courtesy:

1. PW31的“苏木精 - 紫罗兰” - 自己的工作(CC BY 4.0)viaCommons Wikimedia
2. “Haematoxylin powder”By Waughd – Own work(CC BY-SA 4.0)viaCommons Wikimedia