Difference Between Fluorophore and Chromophore

Thekey differencebetween fluorophore and chromophore is thatfluorophore is afluorescentchemical compound whereas chromophore is not a fluorescent compound. It is a part of a chemical compound that is responsible for the color in that molecule.

There are many applications of fluorophores due to its ability to re-emit light upon excitations that occur due to a light source. Some applications include using as a dye or staining agent, as a substrate for enzymes, as a tracer in fluids, etc.

1.Overview and Key Difference
2.What is Fluorophore
3.What is Chromophore
4.Side by Side Comparison – Fluorophore vs Chromophore in Tabular Form
5.概括

What is Fluorophore?

A fluorophore is a fluorescent chemical compound that can re-emit light upon excitations that occur due to a light source. These compounds get this property due to the presence of severalaromatic groups,相互结合或平面/ cycli吗c molecules with several pi bonds (double bonds). These compounds can absorb light energy (of a certain wavelength) and can re-emit this energy as a longer wavelength.

The wavelengths that are absorbed by these compounds depends on the chemical structure of the fluorophore. Usually, these compounds are small, organic compounds, but there can be larger compounds as well. Ex: proteins like green florescent protein.

Difference Between Fluorophore and Chromophore

Figure 01: Fluorescence under UV Radiation

Examples of Flurophore

Some common examples for fluorophores are as follows:

Xanthene derivatives such as fluorescein
氰胺
Naphthalene derivatives
Coumarin derivatives
Pyrene derivatives such as cascade blue
Anthracene derivatives

What is Chromophore?

Chromophore is a part of a molecule, which is responsible for the color of that molecule. This region of molecules has an energy difference between two separate molecular orbitals which falls within the wavelength range of the visible spectrum. Then, when visible light hits this region, it absorbs the light. This causes the excitations of electrons from a ground state to an excited state. Therefore the color that we see is the color that is not absorbed by the chromophore.

在生物分子中，发色团是一个区域，在被光击中时会经历分子的构象变化。共轭PI系统通常用作发色团。共轭PI系统具有单键和双键，以交替的方式。这些系统通常出现在芳香族化合物中。

What is the Difference Between Fluorophore and Chromophore?

A fluorophore is a fluorescent chemical compound that can re-emit light upon excitations that occur due to a light source. Chromophore is a part of a molecule that is responsible for the color of that molecule. This is the main difference between fluorophore and chromophore.

概括– Fluorophore vs Chromophore

荧光团和发色团是负责化合物可见作用的化学物种。荧光团和发色团之间的差异是荧光团是一种荧光化合物，而发色团不是荧光化合物。

Reference:

1. “Fluorophore.” Wikipedia, Wikimedia Foundation, 10 June 2018.Available here
2. “Chromophore.” Wikipedia, Wikimedia Foundation, 10 Apr. 2018.Available here