有限和连续细胞系之间的差异|比较类似术语之间的差异

Thekey differencebetween finite and continuous cell lines is that the有限细胞系进行的确切数量细胞分裂while the continuous cell lines undergo an indefinite number of cell divisions.

Use of primary cell cultures varies according to research purposes. Also, transforming the primary cell cultures intocell linesis necessary to maintain them. Creation of a cell line is possible by sub-culturing the primary cell culture. Furthermore, there are two main types of cell lines depending on their lifespan. Namely, they are finite cell lines and continuous cell lines. Finite cell lines are cell lines that have a definite number of divisions. Consequently, they undergo senescence after a pre-defined number of cell divisions. On the other hand, continuous cell lines are cell lines that have the ability to divide indefinitely. These are immortal cells. Thus, Finite and Continuous cell lines differ from each other mainly by the number of divisions.

内容

1.Overview and Key Difference
2.世界卫生大会t are Finite Cell Lines
3.世界卫生大会t are Continuous Cell Lines
4.Similarities Between Finite and Continuous Cell Lines
5.Side by Side Comparison – Finite vs Continuous Cell Lines in Tabular Form
6。概括

世界卫生大会t are Finite Cell Lines?

Finite cell lines are the primary cell cultures that undergo a definite number of cell divisions before their senescence. They undergo programmed cell death, which is a natural phenomenon. Moreover, finite cell lines are anchorage-dependent cells. They grow inmonolayer cultures。因此，用于维持原代细胞培养物的培养基对其生存至关重要。同样，它包含细胞的所有必要营养素。另外，所有最佳条件都应该在那里供主要细胞系生长良好。

Figure 01: Cell Lines

Besides, the lifespan of finite cell lines depends on the type of cell, type of species, type of cell lineage and the cell culture conditions. Thus, different finite cell lines have a different number of divisions. Usually, the finite cell lines divide about 20 – 100 times before senescence. In the case of humans, human finite cell lines divide about 50 – 100 times before senescence.

世界卫生大会t are Continuous Cell Lines?

Continuous cell lines are cell lines that have the ability to stay immortal. Therefore, these cell lines grow indefinitely. During the primary cell culturing, the cells convert into immortal cells calledtransformants。转化的细胞获得了保持不朽和变性的特殊能力。连续细胞系不依赖锚固，它们具有快速的细胞分裂速率。这些细胞系通常存在于suspension cultures。

Moreover, during the culturing of continuous cell lines, it is necessary to supply medium constituents continuously and to change the media. Also, contaminations should be minimal during the continuous cell line growth. Furthermore, the most often, the preparation of the continuous cell lines is by mutating the cells with carcinogens or infecting the cells with oncogenic viruses. These transform the cells into immortal types.

Above all, the continuous cell lines are said to be more advantageous than the finite cell lines as it’s possible to maintain the continuous cell lines for a longer period of time.

有限和连续细胞系之间有什么相似之处？

Both cell lines are derived from primary cell cultures.
同样，有限和连续细胞系都用于研究目的体外studies.

世界卫生大会t is the Difference Between Finite and Continuous Cell Lines?

有限和连续的细胞系是我们在研究项目中使用的两个细胞系。有限和连续细胞系之间的关键区别在于它们的寿命。有限的细胞系的寿命有限，而连续细胞系的寿命不确定。这是由于以下事实：有限的细胞系具有预定义或预编程的寿命，该寿命限制了它们可以进行的细胞分裂数量。但是，连续细胞系没有编程数量的单元格分裂。因此，连续细胞系可以经历不确定的细胞分裂。在细胞分裂的结尾，有限的细胞系失去了增殖的能力。结果，它们受到衰老。相反，连续细胞系不会失去其增殖的能力。因此，它们无限期地分裂。 Therefore, this also contributes to the difference between finite and continuous cell lines.

Also, we can identify some more differences between the two. The below infographic gives full detils of the difference between finite and continuous cell lines.

摘要 - 有限与连续细胞系

在生物技术研究中，可以使用适当的培养基开发细胞系。而且，这些细胞系有两种主要类型。也就是说，它们是有限且连续的细胞系。原发性细胞培养是两种细胞系中的起始培养物。但是，有限的细胞系具有确定数量的细胞分裂。在经历衰老之前，它大约有20-100个细胞分裂。但是，连续细胞系具有无限数量的细胞分裂。此外，连续细胞培养物主要是致癌的，在准备它们时，有必要将细胞转变为不朽的细胞。因此，这是有限和连续细胞系之间的差异。