Key Difference – Flow Cytometry vs FACS
In the context of cell theory, cells are the basic structural and functional unit of all living organisms. Cell sorting is a methodology that is utilized to separate different cells according to the physiological and morphological features. They can be of intracellular or extracellular characteristics. Interaction ofDNA，，，，RNA， 和蛋白质被认为是细胞内相互作用，而形状，大小和不同的表面蛋白被认为是细胞外特性。在现代科学中，细胞分选方法导致了生物学研究和通过医学研究建立新原则的不同研究。细胞分类是针对各种方法进行的，这些方法包括使用较少的设备和先进的技术方法，并使用复杂的机械。流式细胞仪，荧光激活的细胞分选(FACS), magnetic cell selection and single cell sorting are the major methodologies utilized. Flow cytometry and FACS are developed to differentiate cells according to their optical properties. FACS is a specialized type of flow cytometry。流式细胞仪是一种在分析A分析过程中使用的方法异质细胞的种群根据不同的细胞表面分子，大小和体积，允许研究单细胞。FACS是一个过程，通过将细胞的样品混合物根据其光散射和荧光特性分类为两个或多个容器。这是关键区别在流式细胞仪和FACS之间。
What is Flow Cytometry？
Flow cytometry is a method which is used to examine and determine the expression of intracellular molecules and the cell surface and to define and characterize distinct cell types. It is also used in determining the cell volume and cell size and to evaluate the purity of subpopulations which are isolated. This allows the multi-parameter evaluation of single cells at about the same time. Flow cytometry is used in measuring the intensity of fluorescence which is produced due to fluorescently labeled抗体which help to identify proteins or ligands that bind to associated cells.
一般来说,流式细胞术包括三个子体制ms mainly. They are the fluidics, the electronics, and the optics. In flow cytometry, five main components are available which are used in cell sorting. They are, a flow cell (a stream of liquid that is used to transport them and align the cells for optical sensing process), a system of measurement (can be of different systems including, mercury and xenon lamps, high power water-cooled or low power air-cooled lasers or diode lasers), an ADC; Analog to Digital Converter system, amplification system and a computer for analysis. The acquisition is the process by which the data is collected from the samples using flow cytometer. This process is mediated by a computer which is connected with the flow cytometer. The software present in the computer analyzes the information fed to the computer from the flow cytometer. The software also has the ability in adjusting parameters of the experiment controlling the flow cytometer.
What is FACS?
In the context of Flow cytometry,Fluorescence-activated cell sorting(FACS) is a method which is utilized in differentiating and sorting of a sample of a mixture of biological cells. The cells are separated from two or more container. The sorting method is based on the physical features of the cell which includes light scattering and fluorescence characteristics of the cell. This is an important scientific technique, which can be utilized to obtain reliable quantitative and qualitative results of fluorescence signals that are emitted from each cell. During FACS, initially, the pre-obtained mixture of cells; a suspension is directed to the center of a narrow stream of liquid which is flowing swiftly. The flow of liquid is designed in order to separate the cells in the suspension based on the diameter of each cell. A mechanism of vibration is applied to the stream of suspension which results in the formation of individual droplets.
The system is calibrated in order to create a single droplet with one cell. Just before the formation of droplets, the flow suspension moves along a fluorescence measuring apparatus which detects the fluorescence characteristic of each cell. At the point of formation of droplets, an electrical charging ring is placed which a charge is induced to the ring prior to the measurement of the intensity of fluorescence. Once the droplets are formed from the suspension stream, a charge is trapped within the droplets which then enters into an electrostatic deflection system. According to the charge, the system diverts the droplets into different containers. The method of application of the charge varies according to different systems utilized in FACS. The equipment used in FACS is known as a fluorescence activated cell sorter.
摘要 - 流Cytometry vs FACS
- 流式细胞仪(FCM) /FACS | Fluorescence-Activated cell sorting (FACS). Accessed 22 Sept. 2017.在这里可用
- 同时F。易卜拉欣,蒙古包van den恩格。“流Cytometry and Cell Sorting.” SpringerLink, Springer, Berlin, Heidelberg, 1 Jan. 1970. . Accessed 22 Sept. 2017.在这里可用
- Kierano的“细胞仪” - 自己的工作，（CC由3.0）通过下议院维基梅迪亚
- ‘Fluorescence Assisted Cell Sorting (FACS) B’By SariSabban – Sabban, Sari (2011) Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high- affinity FcεRI receptor (PhD thesis), The University of Sheffield,（CC BY-SA 3.0）通过下议院维基梅迪亚