生化和基于细胞的测定之间的差异

Thekey difference在生化和基于细胞的测定之间是生化测定是基于目标的测定基于细胞assaysarephysiology-based assays.

Assaysare important in research and development of drugs. It is an investigative procedure in the fields of pharmacology, laboratory medicine, and molecular biology for quantitative measurement and qualitative assessment of the presence, quantity, and functional activity of a targeted entity. Hence, biochemical assays and cell-based assays are two types of assays which are used in drug development. Biochemical assays are important in detection and quantification of biological molecules with respect to their activity. On the other hand, cell based assays are important to obtain biologically relevant information to predict and identify the level of response of an organism for a particular substance/drug.

内容

1.Overview and Key Difference
2.What are Biochemical Assays
3.什么是基于细胞的测定
4.Similarities Between Biochemical and Cell Based Assays
5。Side by Side Comparison – Biochemical vs Cell Based Assays in Tabular Form
6。概括

什么是生化测定？

Biological assays are biochemical tools for analyzing biomolecules quantitatively or qualitatively. Hence, they are used to detect and quantify major cellular processes such as细胞凋亡，细胞信号传导和代谢反应。换句话说，在表征它的同时了解目标生物分子是一种常见的生化程序。因此，在药物开发期间，生化主义者利用数百种生化测定法对生物分子进行定量和质量分析。

基于检测，有三种类型的生化测定：比色（成色测定），荧光测定法（荧光）测定和发光测定。在colorimetric assays，可以检测到可见的颜色变化，同时荧光测定, it is possible to detect the emission signals through excitation by a light source. Finally,luminescent assaysdetect the light emitted by a chemical reaction.

什么是基于细胞的测定?

Cell based assays are physiology-based assays that allow the detection of the response of biological organisms to a particular substance or biomolecule. Hence, cell based assays are important in the development of drugs. These assays are体外执行的程序cell cultures。通过基于细胞的测定，调节gene expression可以检测到对特定物质的生物过程的启动或抑制。

The parameters checked by cell-based assays include apoptosis, cell proliferation, oxidative stress, cytotoxicity, cell adhesion, migration, transformation, invasion, and immortalization. Therefore, based on these parameters, there are many types of cell-based assays. They are cell viability assays, cell proliferation assays, cytotoxicity assays, cell senescence assays, and cell death assays.

What are the Similarities Between Biochemical and Cell Based Assays?

• Biochemical and cell-based assays are two types of体外
• 两种类型都有助于检测生物分子在药物开发过程中对生物系统的影响。

生化和基于细胞的测定有什么区别？

Biochemical assays are target based assays while cell based assays are physiology based assays. Thus, this is the key difference between biochemical and cell based assays. Functionally, biochemical assays analyze the activity of bio-molecules quantitatively and qualitatively while cell based assays detect the response of particular organisms to a particular substance or a drug. Hence, this is the functional difference between biochemical and cell based assays.

下面的图表总结了起不同的感情n biochemical and cell based assays.

概括– Biochemical vs Cell Based Assays

Assays are analytical tools for quantitative and qualitative assessment of a targeted molecule/ substance in the context of presence, quantity and functional activity. Hence, they are important in the development of drugs. Biochemical and cell based assays are two such assays useful in the fields of pharmacology and molecular biology. The key difference between biochemical and cell based assays is the type of process and measurement. That is; the biochemical assays are target based while cell based assays are physiology based.

Reference:

1.“基于细胞的测定或生化测定？现在，这就是一个问题……”基于细胞的测定或生化测定，现在问题 - 恩佐生活科学，2019年2月12日，Available here。
2.“TechNote: What Are the Different Types of Cell-Based Assays?” What Are the Different Types of Cell-Based Assays – Enzo Life Sciences, 12 Feb. 2019,Available here。

Image Courtesy:

1. David Muelheims（德国DavidMülheims）的“ Luminol2006” - 自相演(CC BY-SA 2.5)viaCommons Wikimedia
2. “Resazurin assay for mammalian cell viability” By – TwoOars – I (- TwoOars) created this work entirely by myself.,(CC BY-SA 3.0)viaCommons Wikimedia