What is the Difference Between HEK293 and HEK293t

TheHEK293和HEK293T之间的关键区别is that HEK293 is an immortalized cell line derived from the embryonic human kidney that is transfected with sheared humanadenovirustype 5DNA while HEK293t is a女儿牢房line derived from HEK293 original cell line that is transfected with aplasmid vectorcarrying SV40 origin of replication.

Transfection is usually defined as the process of introducing foreign DNA and RNA into cells in order to influence their genotype and phenotype. Such introductions of foreign nucleic acids through various biological, chemical, and physical methods can change the properties of the cell. Ultimately, this allows the study of gene function and protein expression in the context of the cell. HEK293 and HEK293t are two cell lines that have been extensively used in cell biology research for many years because of their propensity for transfection.

内容

1.Overview and Key Difference
2.What is HEK293
3.什么是HEK293T
4.相似之处 - HEK293和HEK293T
5.HEK293vs HEK293t in Tabular Form
6。Summary – HEK293 vs HEK293t

What is HEK293?

HEK293是一种永生的细胞系，衍生自胚胎人肾脏，被剪切的人腺病毒型5DNA转染以产生许多重组蛋白。HEK293最初是由荷兰生物学家Alex van der eb于1973年从人类肾细胞中分离出来的。这些细胞在雌性胎儿的组织培养中生长。后来，他们被范德伯（Van der Eb）实验室的博士后研究员弗兰克·格雷厄姆（Frank Graham）用剪切的人类腺病毒型5DNA转染。该单元线被命名为HEK293，因为这是弗兰克的293^rdexperiment.

The incorporation of adenoviral genes into the HEK cell genome enables these cells to produce a high amount of recombinant proteins very efficiently. Moreover, the adenoviral vector contains the CMV promoter region; hence, it increases this efficiency further. This cell line has been used as a host for gene expression studies. This cell line is also used by the biotechnology industry to produce therapeutic proteins for gene therapy.

什么是HEK293T?

HEK293t is a daughter cell line derived from the HEK293 original cell line that is transfected with a plasmid vector carrying SV40 origin of replication to produce a high amount of recombinant proteins. HEK293t is a human cell line that expresses a mutant version of the SV40 large T antigen. HEK293t was created in Michele Carlos’s laboratory at Stanford through the stable transfection of the HEK29E cell line with a plasmid encoding a temperature-sensitive mutant of the SV40 large T antigen. It was originally referred to as 293/tsA1609neo. This is because the transfection used to create a cell line that confers neomycin resistance and expression of the tsA 1609 allele of SV40 large T antigen.

Due to the expression of a mutant version of SV40 large T antigen, the transfected plasmids with SV 40 origin of replication can greatly increase the amount of recombinant protein. This cell line is commonly used in the biotechnological industry for protein expression and the production of recombinant retroviruses.

What are the Similarities BetweenHEK293 and HEK293t?

• HEK293and HEK293t are two cell lines that have been extensively used in cell biology research because of their propensity for transfection.
• Both cell lines are laboratory-derived.
• 这些细胞系可以大大增加重组蛋白产生的量。
• They are made through transfecting embryonic human kidney cells with a plasmid vector.

HEK293和HEK293T有什么区别？

HEK293是一种永生的细胞系，该细胞系来自胚胎人类肾脏，被剪切的人腺病毒型5DNA转染。相比之下，HEK293T是源自HEK293原始细胞系的子细胞系，该细胞系被带有SV40复制起源的质粒矢量转染。因此，这是HEK293和HEK293T之间的关键区别。此外，HEK293不会表达SV40大T抗原的突变版。另一方面，HEK293T表示SV40大T抗原的突变版。

以下图表列出了差异tween HEK293 and HEK293t in tabular form for side by side comparison.

Summary – HEK293 vs HEK293t

HEK293and HEK293t are two cell lines that have been extensively used in cell biology research because of their steady growth and propensity for transfection. HEK293 is an immortalized cell line derived from an embryonic human kidney that is transfected with sheared human adenovirus type 5DNA, while HEK293t is a daughter cell line derived from HEK293 original cell line that is transfected with a plasmid vector carrying SV40 origin of replication. Thus, this summarizes the difference between HEK293 and HEK293t.

Reference:

1.“”HEK293：具有意外起源的必不可少的人类细胞系。”Lab Pilot: The Laboratory Information Management Blog.
2. Bae, Dahae Hailey, et al. “Design and Testing of Vector-Producing hek293t Cells Bearing a Genomic Deletion of the sv40 t Antigen Coding Region。”分子疗法。方法与临床发展，美国基因与细胞疗法学会，2020年7月9日。

Image Courtesy:

1.“”HEK293“ 经过T. Demont(CC BY-SA 3.0)via Commons Wikimedia
2. “HEK293T中的GFP“ 经过jeremy Seto(CC BY-NC-SA 2.0)via Flickr