这关键区别酶活性和特定活性之间酶活性是指substrates每单位时间转换为产品,而酶的特定活性是指每毫克酶的活性蛋白质。
最多酶是蛋白质分子。它们是生物催化剂和辅助生物学功能。酶通过增加生化反应的反应速率来起作用。此外,它们具有特定的3D形状,可促进其功能。同样,在酶动力学中,酶的行为可以在两个主要测量中表达。它们是酶的酶活性和特异性活性。
CONTENTS
1。概述和关键差异
2。What is Enzyme Activity
3。What is Specific Activity
4。酶活性与特定活性之间的相似性
5。并排比较 - 酶活性与表格形式的特定活性
6.Summary
什么是酶活性?
酶活性将底物的摩尔数定义为每单位时间转换为产品的摩尔数。因此,酶活性测量反应中存在的活性酶摩尔的数量。然而,该测量取决于许多因素,例如温带,pH,底物浓度,酶浓度,抑制剂的存在和激活剂的存在等。。因此,重要的是要保持酶活性达到最佳状态。
在一般情况下,底物和酶的浓度增加可能会增加酶的活性。此外,抑制剂的存在可以阻碍酶活性。相反,诸如二价离子之类的激活的存在可能会诱导反应,同时增加酶的活性。酶活性的SI单位是Katal。但是,由于实用的限制,常用的单位是等于1μmolmin的酶(U)-1。这refore, the enzyme units suggest the activity.
什么是特定活动?
酶的比活性定义了蛋白混合物中酶的纯度。它测量了一毫克总蛋白质中酶的活性。因此,为了评估酶的纯度,该单元在纯化蛋白质的纯化中尤其重要。此外,特定活性是通过在特定条件下(每毫克总蛋白质)在特定条件下形成的产物数量来衡量的。
此外,酶的比活性取决于酶的生化性质以及酶和周围蛋白质的分子间相互作用。特定活动的SI单位是Katal KG-1。但是,由于与该单元相关的实际约束,它表示为μmolmg-1最小-1。此外,酶的特定活性还测量了酶的周转数。
酶活性和特定活性之间有什么相似之处?
- 酶活性和特定活性是两个测量,可评估酶将底物转换为每单位时间的产品的能力。
- 这两个单位都是katal。
- 两者都取决于底物和酶浓度。
What is the Difference Between Enzyme Activity and Specific Activity?
In enzyme kinetics, enzyme activity and specific activity are two important measurements. Enzyme activity measures the rate of substrates converting into products while specific activity measures the activity of an enzyme in one milligram of total protein. Hence, this is the key difference between enzyme activity and specific activity. Furthermore, there is another difference between enzyme activity and specific activity based on the additional use of the specific activity. That is, the specific activity can describe the purity of the enzyme as well.
摘要 - 酶活性与特定活性
酶是一个重要的组件在所有生化reactions. And the efficiency of an enzyme is dependent on its activity. Hence, enzyme activity is the rate at which substrates are converted to products. This implies the efficiency of the enzyme. In contrast, the specific activity is a type of enzyme activity. This signifies the purity of an enzyme per milligram of protein. Nevertheless, both measurements help to analyze the kinetic behaviour of the enzyme. Hence, this is the difference between enzyme activity and specific activity.
参考:
1。Phillips, Theresa. “Why Specific Activity Is Important to Know When Purchasing Enzymes.” The Balance Small Business, The Balance.在这里可用
图片提供:
1. wladimir labeikovsky的“酶高压b” - flickr,(CC BY-SA 2.0)通过下议院维基梅迪亚
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