的区别是tween 1D and 2D Gel Electrophoresis

Thekey differencebetween 1D and 2D gel electrophoresis is the properties used for the separation ofproteins上gel electrophoresis.1D gel electrophoresis only separates proteins based on the分子量while 2D gel electrophoresis separates proteins based on its iso-electric point and molecular weight.

Protein separation by gel electrophoresis is an important technique to characterize proteins. Proteins have varying properties; therefore, separation is more complex in comparison to DNA separation by Agarose gel electrophoresis.

内容

1.Overview and Key Difference
2.What is 1D Gel Electrophoresis
3.什么是2D凝胶电泳
4.1D和2D凝胶电泳之间的相似性
5.Side by Side Comparison – 1D vs 2D Gel Electrophoresis in Tabular Form
6.概括

什么是1D凝胶电泳？

1D Gel Electrophoresis, also known as one dimension gel electrophoresis, is a method of protein separation based on the molecular weight. Protein separation mainly takes place using polyacrylamide gel electrophoresis. Based on the concept of gel electrophoresis, molecules separate on their property of molecular weight and charge.

因此，为了给蛋白质均匀电荷，十二烷基硫酸钠（SDS）处理是在凝胶电泳之前进行的。SDS贬低蛋白质，并在蛋白质上提供均匀的负电荷；当发生电场的应用时，蛋白质会根据其分子量迁移到阳性末端。因此，在分离中，仅考虑一个财产。这就是为什么该方法称为1D凝胶电泳的原因。

During 1D gel electrophoresis, proteins are separated based on their molecular weight. In this regard, the lower weight molecules migrate faster in the gel compared to the high molecular weight proteins. Thus, high weight proteins remain close to the wells.

什么是2D凝胶电泳?

2D凝胶电泳或二维凝胶电泳根据两种特性分离蛋白质。这两个属性是iso-electric pointof the protein and molecular weight. This method of protein separation increases the resolution of protein separation. The iso-electric point of the protein depends on the pH at which the protein is neutral.

Thus, in 2D gel electrophoresis, the protein is allowed to run on a fixed pH gradient in the first dimension. In the second dimension, the proteins are separated using vertical or horizontal polyacrylamide gel electrophoresis. Thus, the proteins separate according to their molecular weight in the second dimension.

此外，这种凝胶电泳方法增加了蛋白质分离的分辨率。因此，分离的蛋白质更纯化。但是，该技术的成本远高于一维凝胶电泳。

What are the Similarities Between 1D and 2D Gel Electrophoresis?

• Both techniques separate proteins.
• Hence, they are important in characterizing proteins.

What is the Difference Between 1D and 2D Gel Electrophoresis?

The key difference between 1D and 2D gel electrophoresis is that 1D gel electrophoresis separates proteins based only on the molecular weight while 2D gel electrophoresis separates proteins based on both iso-electric point and molecular weight. Due to this basic difference between 1D and 2D gel electrophoresis, the resolution of separation of the proteins and the cost of the two techniques also vary. 2D gel electrophoresis shows high resolution than 1D gel electrophoresis. However, 2D gel electrophoresis is more costly than 1D gel electrophoresis.

Below infographic summarizes the difference between 1D and 2D gel electrophoresis.

概括– 1D vs 2D Gel Electrophoresis

蛋白质的分离取决于许多因素。1D凝胶电泳仅基于分子量分离蛋白质。但是，二维或2D凝胶电泳会增加蛋白质分离的分辨率。此外，2D凝胶电泳根据ISO-电点和分子量分离蛋白质。因此，这些数据对于蛋白质和蛋白质组学领域的下游加工很重要。因此，这是1D和2D凝胶电泳之间差异的摘要。

Reference:

1. Galeva，Nadezhda和Michailaltermann。“比较一维和二维凝胶电泳作为大鼠肝微粒体蛋白质组学分析的分离工具：细胞色素P450和其他膜蛋白。”蛋白质组学，美国国家医学图书馆，2002年6月，Available here.

Image Courtesy:

1. “Electrophoresis – Filling 1D gel wells with proteins mixture” By Jean-Etienne Poirrier – Filling 1D gel wells with proteins mixture(CC BY-SA 2.0)viaCommons Wikimedia
2. “2D electrophoresis” By National Institute of Health – Center for Cancer Research Nanobiology Program, National Institute of Health (Public Domain) viaCommons Wikimedia