稳态和时间分辨荧光有什么区别| Compare the Difference Between Similar Terms

Thekey difference between steady state and time resolved fluorescenceis that the steady-state fluorescence involves the study of long-term average fluorescence of a sample when irradiated with UV, visible or near IR light, whereas the time-resolved fluorescence involves the study of fluorescence of a sample that is monitored as a function of time after the excitation by a light pulse.

Fluorescence can be defined as the visible or invisible radiation that emits from a substance due to theincident radiation短波长。换句话说，它是通过吸收光或其他类型EMR的物质发射光的（电磁辐射). The most common example for fluorescence is the absorption of radiation in the UV region of the spectrum by a sample (which is invisible to us) and emitting the light in the visible region. This gives the sample a distinct color that is observable only upon UV light. Moreover, fluorescent materials tend to glow nearly immediately upon the removal of the radiation source.

CONTENTS

1.Overview and Key Difference
2.What is Steady-State Fluorescence
3.What is Time-Resolved Fluorescence
4.稳态与时间分辨的荧光以表格形式
5.概括

What is Steady-State Fluorescence?

A steady-state fluorescence is an analytical technique that studies the long-term average fluorescence of a sample upon the irradiation of that sample with UV, visible, or near IR light. The applications of steady-state fluorescence includeexcitation and emissionscans, synchronous scans and maps, steady-state fluorescence anisotropy, excitation-emission maps, kinetic measurements, and temperature maps.

What is Time-Resolved Fluorescence?

A time-resolved fluorescence is an analytical technique that studies the fluorescence of a sample that is monitored as a function of time after the excitation by a light pulse. We can name it an extension of fluorescence spectroscopy. In this technique, we need to monitor a sample (after its excitation through a flash of light) as a function of time.

有不同的方法,我们可以获得一个时间-resolved fluorescence, including fast-detection electronics, time-correlated single-photon counting, a streak camera, intensified CCD cameras, optical gating, etc. During the time-resolved fluorescence, the convolution integral is used to calculate a lifetime from fluorescence decay.

稳态和时间解析荧光有什么区别？

荧光可以定义为由于短波长的入射辐射而从物质中散发出的可见或看不见的辐射。荧光有两个衍生物作为稳态和时间分辨荧光。稳态和时间分辨荧光之间的关键区别在于，当用紫外线，可见或接近红外光照射时，稳态荧光涉及样品长期平均荧光的研究，而时间分辨荧光涉及研究的研究。样品的荧光由光脉冲激发后的时间函数监测。

A steady-state fluorescence is used in excitation and emission scans, synchronous scans and maps, steady-state fluorescence anisotropy, excitation-emission maps, kinetic measurements, and temperature maps. A time-resolved fluorescence, on the other hand, is used in TR-FRET systems (time-resolved fluorescence energy transfer)

The following table summarizes the difference between steady state and time resolved fluorescence.