Thekey differencebetween gas chromatography and mass spectrometry is thatgas chromatography is important in separating components in a mixture, whereas mass spectrometry is useful in calculating the exact molecular weight of the sample components.
一般来说,使用气相色谱法和mass spectrometry because we can separate components in a mixture using gas chromatography and can identify those components using mass spectrometry.
内容
1.Overview and Key Difference
2.什么是气相色谱
3.What is Mass Spectrometry
4.Side by Side Comparison – Gas Chromatography vs Mass Spectrometry in Tabular Form
6。概括
什么是气相色谱?
Gas chromatography is an analytical technique in which a移动阶段和固定阶段is used where the mobile phase is in the gas state. A chromatographic technique is an analytical test used to separate, identify, and sometimes quantify the components in a mixture. There are two types as gas-solid chromatography and gas-liquid chromatography.
In gas-solid chromatography, the stationary phase is in the solid-state and the mobile phase is in the gaseous state. Here, gas-solid chromatography is used for the separation of volatile components in a mixture. This technique includes both the mixture and the mobile phase in the gaseous state. The mobile phase and the mixture we are going to separate combine with each other, and then this mixture passes through the solid stationary phase. The stationary phase is applied to the inner wall of a tube known as the chromatographic column. The molecules of the stationary phase can interact with the molecules in the mobile phase.
In gas-liquid chromatography, the stationary phase is in the liquid state while the mobile phase is in the gaseous state. There, the stationary phase is a nonvolatile liquid. We need to apply this stationary phase on the inner wall of a tube known as the chromatographic column. Then, the inner wall act as a solid support for the stationary phase. In this technique, the mobile phase is an inert gas such as Argon, Helium, or Nitrogen.
什么是质谱?
质谱法(通常用MS表示)是一种分析化学技术,可测量离子的质量与电荷比。该技术的最终结果是作为强度图的质谱。此外,我们需要绘制此图作为质量到电荷比的函数。在质谱法中,我们用于测量的仪器是质谱仪。当我们将样品引入该仪器时,样品分子会经历电离。在这种电离过程中,选择适当的电离技术非常重要,因为它对最终结果产生了很大的影响。如果我们使用试剂气体,例如氨将导致样品分子的电离仅形成正离子或仅由负离子形成,具体取决于仪器的设置。
质谱中的阳性电离涉及形成阳性离子,以测定样品分子的质量与电荷比。我们称此为正离子模式在质谱法中。我们可以将这种阳性离子表示为M-H+。In this technique, we can detect ions in a high yield.
质谱法的负电离涉及为测定样品分子质量与电荷比的负离子的形成。我们称此为负离子模式在质谱法中。此外,我们可以将这种负离子表示为M-H–。In this technique, we can detect these ions in a high yield.
What is the Difference Between Gas Chromatography and Mass Spectrometry?
Gas chromatography is an analytical technique in which a mobile phase and a stationary phase is used where the mobile phase is in a gas state. Mass spectrometry (often denoted by MS) is a technique in analytical chemistry that measures the mass-to-charge ratio of ions. Therefore, the key difference between gas chromatography and mass spectrometry is that gas chromatography is important in separating components in a mixture, whereas mass spectrometry is useful in calculating the exact molecular weight of the sample components.
The below infographic summarizes the difference between gas chromatography and mass spectrometry in tabular form.
概括– Gas Chromatography vs Mass Spectrometry
Often we are using gas chromatography followed by mass spectrometry in order to separate the components in a desired mixture followed by their identification. The key difference between gas chromatography and mass spectrometry is that gas chromatography is important in separating components in a mixture, whereas mass spectrometry is useful in calculating the exact molecular weight of the sample components.
Reference:
1.“气相色谱”。化学LibreTexts, Libretexts, 15 Aug. 2020,Available here。
Image Courtesy:
1. EN:用户:rune.welsh - 自己的作品的“气相色谱”(CC BY-SA 3.0)viaCommons Wikimedia
2. “Mass spectrometry protocol” By Philippe Hupé – Emmanuel Barillot, Laurence Calzone, Philippe Hupé, Jean-Philippe Vert, Andrei Zinovyev, Computational Systems Biology of Cancer Chapman & Hall/CRC Mathematical & Computational Biology , 2012(CC BY-SA 3.0)viaCommons Wikimedia
发表评论