Key Difference – Adsorption vs PartitionChromatography
吸附色谱和分隔色谱是色谱的品种。吸附色谱法通过吸附分离化合物,而分区色谱法则通过分区分离化合物。This is thekey differencebetween adsorption chromatography and partition chromatography.
Chromatography is a laboratory technique that is utilized in the context of separation of mixtures. It consists of two phases namely mobile phase and stationary phase. The stationary phase of adsorption chromatography is in a solid state while, in partition chromatography, the stationary phase is in a liquid state.
内容
1.Overview and Key Difference
2.What is Adsorption Chromatography
3.What is Partition Chromatography
4.Similarities Between Adsorption and Partition Chromatography
5.Side by Side Comparison – Adsorption vs Partition Chromatography in Tabular Form
6。概括
What is Adsorption Chromatography?
Adsorption chromatography is defined as a type of chromatography where the solute molecules are directly bound to the surface of the stationary phase. In simple terms, adsorption chromatography could be explained as a gas or liquid that is adsorbed to a surface of a solid. Stationary phases possess a variety of adsorption sites.
这些吸附位点在韧性方面与它们在相对丰度中结合的分子方面有所不同。吸附剂活性取决于净效应。吸附色谱法在固态中使用流动相和气体状态和固定相。每个溶质在吸附到固体表面和溶解度in the solvent. Therefore, the solvent will move up with the mobile phase, and at a point where a state of equilibrium is reached, the solvent will be adsorbed to the stationary phase.
The difference in travelling distances of compounds could be used to identify the particular compound. There are three types of adsorption chromatographic techniques namely, paper chromatography, thin layer chromatography and column chromatography.
What is Partition Chromatography?
Partition chromatography is another type of chromatography that works under the same principle with slight alterations. This particular technique was introduced by Archer Martin and Richard Laurence Millington Synge in the time period of 1940. Similar to other chromatography variances, partition chromatography also contains a stationary phase and a mobile phase.
固定相和移动相都是液体。在液态液体分离期间,当特定化合物达到平衡条件下存在的两个不混溶的液相时,会分离。两个液相是吸附柱中存在的原始溶剂和溶剂膜。
The work of Martin and Synge on partition chromatography has lead to the development of other varieties of chromatography such as gas-liquid chromatography, gas chromatography and paper chromatography. The invention of paper chromatography in later years lead to the development of thin layer chromatography that is an advanced technology based on paper chromatography.
吸附和分区色谱之间有什么相似之处?
- Both adsorption and partition chromatography are varieties of chromatography
- Both types of chromatography function under the same principle of chromatography
- 两种类型都用于分离化合物的混合物。
- Both adsorption and partition chromatography contain stationary and mobile phases.
- Adsorption and partition chromatography possess the ability to separate compounds in all three states; gas, liquid and solid.
- 两种类型的流动阶段在液态状态下。
吸附和分区色谱之间有什么区别?
吸附和分区色谱法 |
|
Adsorption chromatography is defined as a type of chromatography in which separation occurs based on adsorption. | Partition chromatography is a type of chromatography in which separation is based on partition. |
Extraction | |
Adsorption chromatography is a liquid-solid extraction. | Partition chromatography is a liquid-liquid extraction. |
固定阶段 | |
固定相处于吸附色谱的固态。 | The stationary phase is a liquid state in partition chromatography. |
发展 | |
Adsorption chromatography was not further developed. | Partition chromatography leads to the development of other types of chromatography. |
概括–吸附和PartitionChromatography
色谱法是一种用于将化合物与混合物分离的技术。吸附和分隔色谱是两种类型的色谱。吸附色谱的固定相是固态。固定相具有多种吸附位点。在分区色谱法中,固定相处于液态状态。两种类型的流动阶段在液态状态下。吸附和分区色谱之间的差异是分子的分离基于吸附色谱中的吸附能力,而分离基于分区色谱中的隔板进行分离。
参考 :
1.“Partition Chromatography.” Partition Chromatography |[电子邮件保护]Available here
2.“Adsorption Chromatography.” Study.com, Study.com.Available here
Image Courtesy:
1.’Column chromatography (Public Domain) viaCommons Wikimedia
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