FPLC和HPLC有什么区别| Compare the Difference Between Similar Terms

这关键区别FPLC和HPLC是FPLC是一个液相色谱类型which purifies large biomolecules such as proteins, nucleotides and peptides, while HPLC is a type of liquid chromatography that separates small molecular weight compounds.

Liquid chromatography is a technique used to separate a sample into its individual components. This separation occurs due to the interaction of specific samples withmobile and stationary phases。样品中的组件基于每个组件对流动阶段在液相色谱中的亲和力进行分离。当流动相和样品通过列时，样品的成分开始分成带UV-VIS光谱检测的频段。因此，FPLC和HPLC是两种类型的液相色谱技术。

1。概述和关键差异
2。什么是FPLC
3。What is HPLC
4。Similarities – FPLC and HPLC
5.FPLC vs HPLC in Tabular Form
6.摘要 - FPLC与HPLC

什么是FPLC？

FPLC是一种液态色谱法，可净化大型生物分子，例如蛋白质，核苷酸和肽。这fast protein liquid chromatography(FPLC) was first developed and marketed in Sweden by Pharmacia company in 1982. It is often used to analyse or purify protein mixtures. This takes place based on the principle of sample components affinities for moving fluid (mobile phase) and a porous solid material (stationary phase). In FPLC, the mobile phase is a buffer, and the stationary phase is a resin composed of beads. It usually consists of cross-linked agarose packed into a cylindrical glass or plastic column.

Figure 01: FLPC (Fast Protein Liquid Chromatography) Apparatus

在大多数FLPC策略中，都使用离子交换树脂。因此，感兴趣的蛋白质将通过电荷相互作用与树脂结合。FLPC技术还使用两个缓冲区：缓冲区1（运行缓冲区）和缓冲区2（洗脱缓冲区）。最初，当运行缓冲液和样品混合物穿过色谱柱时，混合物中感兴趣的蛋白质将通过电荷相互作用与树脂结合。但是，当洗脱缓冲液在过程结束时穿过柱子时，感兴趣的蛋白质变得分离，并在洗脱缓冲液中返回溶液。后来，溶液（含有感兴趣的蛋白质的洗脱液）通过两个检测器，它们测量了盐浓度和蛋白质浓度。当每种蛋白质洗脱时，它在检测过程中似乎是“峰”，可以收集以供进一步使用。

什么是HPLC？

高效液相色谱是一种液相色谱,国家环保总局rates small molecular weight compounds. In 1969, the first HPLC was commercially manufactured by Waters Corporation, USA. In high-performance liquid chromatography (HPLC), a sample mixture (analyte) in a solvent (mobile phase) is pumped through a column with chromatographic packing material (stationary phase) at high pressure. The sample mixture is carried by a moving carrier gas stream of helium or nitrogen.

HPLC- High-Performance Liquid Chromatography Principle

图02：HPLC（高性能液相色谱）

样品混合物中与固定相或与流动相的相互作用最少相互作用的组件将更快地退出列。另一方面，样品混合物中与固定相或与流动相的相互作用最少相互作用的组件将退出列较慢。基于上述相互作用原理，可以分离样品混合物的成分。此外，仪器检测器可以识别出退出色谱柱的样品混合物的每个组件。HPLC用于检查环境和生物样品是否存在或不存在已知化合物，例如药物，毒素或农药。它用于药物，环境，取证和化学物质等不同行业。

FPLC和HPLC之间有什么相似之处？

FPLC和HPLC是液相色谱的类型。
两者都用于分离和识别生物样品。
它们具有液体流动相和坚固的固定相。
Both techniques use a column to pass through the sample.
这se techniques use pumps, detectors, valves and software for sample separation and detection.

FPLC和HPLC有什么区别？

FPLC是一种液相色谱，用于纯化大型生物分子，例如蛋白质，核苷酸和肽。HPLCis a type of liquid chromatography that is used to separate small molecular weight compounds. So, this is the key difference between FPLC and HPLC. Moreover, FPLC uses pH and conductivity monitors as wells as fraction collectors. In contrast, HPLC does not use pH and conductivity monitors and fraction collectors.

以下信息图列出了表格中FPLC和HPLC之间的更多差异。

摘要 - FPLC与HPLC

色谱法是一种实验室分析技术，用于将组件与混合物分离。它分为几种类型，例如色谱柱色谱，气相色谱，液相色谱，离子交换色谱法等。FPLC和HPLC是两种类型的液相色谱技术。FPLC是一种液相色谱，用于纯化大型生物分子，例如蛋白质，核苷酸和肽。另一方面，HPLC是一种用于分离小分子量化合物的液相色谱。因此，这是FPLC和HPLC之间的差异的摘要。