Difference Between Gel Filtration and Gel Permeation Chromatography

Thekey differencebetween gel filtration and gel permeation chromatography is thatthemobile phase凝胶过滤chromatographyis an aqueous solution whereas the mobile phase of gel permeation chromatography is an organic solvent.

Both gel filtration and gel permeation chromatography come under the category of size exclusion chromatography in which we can separate the molecules in a solution depending on their size. That is, sometimes, we can separate the molecules according to their molecular weights. Usually, we use these techniques to separate complex molecules such as proteins and in industrial scale, to separate polymer materials. The main difference between gel filtration and gel permeation chromatography is in the mobile phase we use in each technique.

内容

1.Overview and Key Difference
2.What is Gel Filtration Chromatography
3.什么是凝胶渗透色谱法
4.并排比较 - 表格形式的凝胶过滤与凝胶渗透色谱法
5.概括

什么是凝胶过滤色谱法？

Gel filtration chromatography is a form of size exclusion chromatography in which we use an aqueous solution as the mobile phase. Therefore, most of the times, the mobile phase that we use in this technique is an aqueous buffer. Also, we use a chromatographic column for this separation, and we need to pack the column with porous beads. Usually, Sephadex andagarose多孔材料是有用的。因此,这些马terial are the stationary phase of our experiment. Moreover, we can use the pore size of these beads to determine the size of macromolecules that we are separating. However, it is just an estimation.

The major application of this technique is to separate proteins and other water-soluble material according to the size. The technique works by trapping small molecules in pores of the stationary phase (beads) while large molecules elute through the gel. Therefore, the first fraction contains large molecules. Then we can use a different solvent which can take out the small molecules inside pores. Then our second fraction contains small molecules.

什么是凝胶渗透色谱法?

凝胶渗透色谱法是一种尺寸排除色谱的一种形式，我们使用有机溶剂作为流动相。因此，我们可以为此目的使用己烷和甲苯等解决方案。

The stationary phase is a porous material same as in gel filtration chromatography. This technique is often applicable to polymers and other organic-soluble material. The method of action of the technique is similar to that of gel filtration chromatography.

What is the Difference Between Gel Filtration and Gel Permeation Chromatography?

Gel filtration and gel permeation chromatography are two forms of size exclusion chromatography that involves in the separation of molecules in a sample according to the size of molecules. The only difference between gel filtration and gel permeation chromatography is in the mobile phase they use and hence, the application of the technique. Therefore, the key difference between gel filtration and gel permeation chromatography is that the mobile phase of gel filtration chromatography is an aqueous solution whereas the mobile phase of gel permeation chromatography is an organic solvent.

The below infographic presents the difference between gel filtration and gel permeation chromatography in tabular form.

概括– Gel Filtration vs Gel Permeation Chromatography

尺寸排除色谱分为两种主要类型，作为凝胶过滤和凝胶渗透色谱法。凝胶过滤和凝胶渗透色谱之间的关键区别在于，凝胶过滤色谱的流动相是水溶液，而凝胶渗透色谱的流动相是有机溶剂。

Reference:

1.“尺寸排斥色谱”。Wikipedia，Wikimedia基金会，2018年9月28日。Available here
2. Ngwa, Asabuwa. “Gel Permeation Chromatography (GPC).” LinkedIn SlideShare, 19 July 2015.Available here

Image Courtesy:

1.”Size exclusion”By Kjaergaard (Public Domain) viaCommons Wikimedia
2.”GPC instrument”By Chem538grp2w09 (talk) – Own work (Public Domain) viaCommons Wikimedia